The Arabidopsis thaliana developmental promoters of WUSCHEL (WUS) and COLD SHOCK DOMAIN PROTEIN 3 (CSP3) were tested for their suitability as potential control elements for the Cre-Lox recombination system in poplar cells. To remove the integrated transgene after CRISPR machinery has modified the genome, timely control of Cre is required....
Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...