The adenovirus replication origins reside in the inverted terminal
repetition (ITR) sequences. Replication proceeds unidirectionally by a
strand-displacement mechanism from either or both origins to produce
duplex daughter DNA plus a displaced parental strand. The displaced
strand can be duplicated by two different routes. The cis pathway involves
intramolecular hybridization...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...
The aim of this study was to gain more information about the
interactions between DNA and the histone octamer during the
process of transcription. This work used a pUC8 plasmid derivative
that contained the core promoter region of the RNA polymerase I of
Acanthamoeba castellanii, placed upstream of four repeats...