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Filtering by: Has journal Cryobiology Remove constraint Has journal: Cryobiology Committee Member McGuire, Joseph Remove constraint Committee Member: McGuire, Joseph

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Optimization of cryoprotectant addition and removal procedures for vitrification of adherent mammalian cells

Creator:

Fry Davidson, Allyson

Abstract:

Cryopreservation of adherent cells may be advantageous for cell types that are difficult to preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...

Resource Type:

Dissertation

Full Text:

governing principle of cryobiology that describes the relationship between the rate of temperature change
Membrane permeability properties of human granulocytes

Creator:

Vian, Alexander M.

Abstract:

In the last decade, there has been renewed interest in the use of granulocyte transfusions to treat infections in individuals with compromised immune systems. However, granulocytes only remain functional for about a day after isolation and this short shelf life is a significant drawback. Cryopreservation would allow long term storage...

Resource Type:

Masters Thesis
Improving the post-thaw processing of cryopreserved red blood cells using a combined approach of mathematical modeling and microfluidics

Creator:

Lusianti, Ratih E.

Abstract:

This study lays the groundwork on potential techniques that could be employed to improve the post-thaw wash processing of cryopreserved human red blood cells. Transfusion of red blood cells is one of the most commonly practiced procedures in clinical medicine. Millions of red blood cell units are transfused to patients...

Resource Type:

Dissertation
Removal of cryoprotectant with the use of a microseparation device

Creator:

Lusianti, Ratih E.

Abstract:

Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing, it is often desirable to remove them before infusion into a patient. Currently, the most common method for CPA removal is by centrifugation. This method...

Resource Type:

Masters Thesis

Full Text:

cryobiology known today as cryopreservation. Cryopreservation is defined as a technique of maintaining

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Academic Affiliation

Chemical, Biological and Environmental Engineering4

Advisor

Higgins, Adam2
Higgins, Adam Z.2

Commencement Year

Commencement Year range begin – Commencement Year range end

Current results range from 2011 to 2014

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Committee Member

McGuire, Joseph[remove]4
Alani, Adam1
Baio, Joe1
Jovanovic, Goran1
Kelly, Christine1
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Creator

Lusianti, Ratih E.2
Fry Davidson, Allyson1
Vian, Alexander M.1

Date

Date range begin – Date range end

Current results range from 2010 to 2014

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Decade

2010-20194

Degree Field

Chemical Engineering4

Degree Level

Doctoral2
Master's2

Degree Name

Doctor of Philosophy (Ph.D.)2
Master of Science (M.S.)2

Language

English [eng]4

License

All rights reserved4

Non-Academic Affiliation

Oregon State University. Graduate School4

Peer Reviewed

No4

Resource Type

Dissertation2
Masters Thesis2

Rights Statement

In Copyright4

Subject

Blood cells -- Cryopreservation2
Glycerin2
Separation (Technology)2
Cell adhesion1
Cells -- Cryopreservation1
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