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Filtering by: Has journal Plant, Cell & Environment Remove constraint Has journal: Plant, Cell & Environment Committee Member Ream, Walt Remove constraint Committee Member: Ream, Walt Degree Field Microbiology Remove constraint Degree Field: Microbiology

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  • Quorum sensing gene regulation in Pseudomonas aeruginosa

    Creator:
    Gupta, Rashmi
    Abstract:
    Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
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    those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell

  • Two new roles for the TYMV tRNA-like structure : translation enhancement and repression of minus strand synthesis

    Creator:
    Matsuda, Daiki
    Abstract:
    Some positive-strand RNA plant viruses possess a transfer RNA-like structure (TLS) at the 3'-terminus of their genomic RNAs. The closest mimicry to tRNA is exhibited by the valylatable TLSs from tymoviruses and furo-like viruses, which are able to interact with key cellular tRNA enzymes: [CTP, ATP]:tRNA nucleotidyltransferase (CCA NTase), valyl-tRNA...
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    approved: Theo W.Dreher Some positive-strand RNA plant viruses possess a transfer RNA-like structure

  • Distribution, decay, and quantification of fecal source-tracking markers

    Creator:
    Green, Hyatt C.
    Abstract:
    Waterborne fecal contamination poses serious risks to human health and can disrupt aquatic ecosystems. Molecular marker methods are widely used to identify and, in some cases, quantify the sources of contamination and guide management decisions regarding water resource protection. However, methods to detect some likely sources of fecal contamination such...
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    environment. The distribution of the markers differed across geographic location and host species. Two

  • Comparative genomics of Vibrio and characterizing microbial communities at a shellfish hatchery

    Creator:
    Delgado, Hanna
    Abstract:
    Vibrio bacteria are commonly found in freshwater, marine, and estuarine environments. Members of this genus can exist in commensal relationships with marine animals or as planktonic bacteria, however, they are well known for their roles as pathogens towards humans and animals. In this dissertation, I investigated aspects of host-bacteria relationships...
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    host environment. Overall, these results highlight specific features that may enable Vibrio pathogen

  • Cooperation and conflict in Pseudomonas aeruginosa quorum sensing

    Creator:
    Wilder, Cara N.
    Abstract:
    Pseudomonas aeruginosa is an environmental bacterium as well as an opportunistic pathogen that primarily infects immunocompromised individuals, including those suffering from cystic fibrosis. The density-dependent regulation of gene expression via cell-to-cell communication, also termed quorum sensing (QS), is an important virulence determinant in this organism. Generally, P. aeruginosa uses three...
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    freely diffuses across the cell membrane into the surrounding environment. Once a critical autoinducer

  • Characterization of Baculovirus genes involved in genome replication and processing

    Creator:
    Vanarsdall, Adam L.
    Abstract:
    The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
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    open reading frames, Ac 101, Ac 142, and Ac 144. Using the Spodoptera frugiperda cell line as a

  • The Fitness Costs and Regulatory Strategies of Cooperative Siderophore Production in Pseudomonas

    Creator:
    Sexton, David
    Abstract:
    Cooperative behaviors in bacteria are increasingly appreciated for their relevance to microbial ecology and utility as model systems for social evolution. One example is the secretion of siderophores, a structurally diverse group of compounds that chelate extracellular iron. Siderophore production is considered cooperative because the benefits can be shared with...
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    from the extracellular environment. This is considered cooperation because the benefits are shared

  • Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

    Creator:
    Quinn, Matthew J.
    Abstract:
    The pathogen Vibrio cholerae uses cations as a primary currency of virulence and environmental persistence, using gradients of those cations to move, acquire nutrients, and control virulence gene expression. An understanding of the overlapping roles of bioenergetics and chemotaxis in the virulence and environmental survival of V. cholerae issues from...
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    homeostasis and growth in an acidic environment. Further, a model system comprised of a V. cholerae strain