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Filtering by: Creator Palo, Daniel R. Remove constraint Creator: Palo, Daniel R. Committee Member Rockey, Daniel Remove constraint Committee Member: Rockey, Daniel Resource Type Dissertation Remove constraint Resource Type: Dissertation

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  • The role of chlamydial inclusion membrane proteins in host-pathogen interaction and the development of novel methods for studying chlamydial biology

    Creator:
    Alzhanov, Damir T.
    Abstract:
    The majority of our modern understanding of bacterial pathogenesis is based on the strategy that involves screening bacterial genomes for the presence of the genes encoding pathogenic factors, and analysis of these genes via forward and reverse genetics. Chlamydiae represent a unique group of pathogenic bacteria in which it is...
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    Daniel D. Rockey The majority of our modern understanding of bacterial pathogenesis is

  • Recombinant vaccines against infectious hematopoietic necrosis virus : bacterial systems for vaccine production and delivery

    Creator:
    Simon, Benjamin E.
    Abstract:
    Several systems were examined for the production and delivery of recombinant vaccines for fish. C. crescentus was employed to produce a fragment of the IHNV glycoprotein. When administered by injection to 0.5 gram rainbow trout (Oncorhynchus mykiss), one of the fusion proteins (184 amino acids of the IHNV glycoprotein fused...
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    graduate committee members, especially Dr. Lyle Brown and Dr. Daniel Rockey, for stimulating discussions

  • New Insights into the Molecular Mechanism of Clostridium perfringens Spore Germination

    Creator:
    Banawas, Saeed S.
    Abstract:
    C. perfringens is a spore-forming, gram-positive, anaerobic pathogenic bacterium capable of causing a wide variety of diseases in both humans and animals. However, the two most common illnesses in humans are C. perfringens type A food poisoning (FP) and non-food-borne (NFB) gastrointestinal (GI) illnesses. Interestingly, these two major diseases are...
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    . CONTRIBUTION OF AUTHORS Chapter 2: Dr. Mahfuzur R. Sarker and Dr. Daniel Paredes-Sabja contributed to the

  • Clostridium perfringens spores : inactivation, germination, and formation

    Creator:
    Udompijitkul, Pathima
    Abstract:
    The enterotoxin-producing Clostridium perfringens type A isolates are responsible for the third most common foodborne illness in the United States and can also cause non-foodborne human gastrointestinal (GI) diseases such as antibiotic- associated and sporadic diarrheas. Three important factors contribute to the ability of C. perfringens to cause GI diseases,...
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    : __________________________________________________________________ Mahfuzur R. Sarker The enterotoxin-producing Clostridium perfringens type A isolates are

  • Evolutionary Genomic Responses to Mitochondrial Dysfunction in Caenorhabditis elegans

    Creator:
    Wernick, Riana I.
    Abstract:
    Understanding the impact of mitochondrial dysfunction on genome evolution has the potential not only to provide new insights on the basic evolutionary processes influencing mitochondrial and nuclear genomes, but may also reveal novel avenues for evolutionary adaptive recovery from harmful mutations. Aberrant mitochondrial activity is fundamental to the pathology of...
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    elegans Abstract approved: ______________________________________________________ Dee R. Denver

  • Structural studies of the antioxidant defense enzymes; copper, zinc superoxide dismutase and alkyl hydroperoxide reductase flavoprotein

    Creator:
    Roberts, Blaine R.
    Abstract:
    Oxygen derived radicals are involved in many aspects of life from aging and cell signaling to disease states as diverse as heart disease, diabetes, neurodegeneration and inflammation. Therefore, understanding the function of antioxidant defense proteins and the effects of oxygen derived radicals on protein function is essential to elucidate the...
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    AN ABSTRACT OF THE THESIS OF Blaine R. Roberts for the degree of Doctor of Philosophy in

  • Investigation of unique marine environments for microbial natural products

    Creator:
    Thornburg, Christopher C.
    Abstract:
    Metagenomics has revealed that the marine microbial biosphere is immensely more diverse than originally considered, and is an almost untapped reservoir for the potential discovery of microbial natural products. Despite numerous advances in culturing, biosynthetic engineering and genomic-based screening efforts to uncover much of this diversity in relatively accessible environments,...
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    external committee members, Dr. Martin Schuster and Dr. Daniel Rockey for your time and assistance during

  • Characterization of an inhibitor ("6S") of infectious pancreatic necrosis virus (IPNV) in normal rainbow trout serum (RTS) and its effects on the virus

    Creator:
    Park, Kyoung Chul
    Abstract:
    The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV) found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a molecular weight of approximately 150 kDa and was dependent on divalent cations, either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
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    -R). Enzyme immunodot assay revealed that Ja-S clones and Ja-R clones differed by several epitopes

  • Syntheses and evaluation of putative enzyme inhibitor of isoprenoid biosynthesis

    Creator:
    Phaosiri, Chanokporn
    Abstract:
    The discovery of the methylerythritol phosphate pathway (the MEP pathway) as an alternate pathway for isoprenoid biosynthesis in some organisms including most bacteria, malarial parasites and plants, but not in animals, has stimulated extensive studies in this area. Research has revealed the potential of finding novel antibacterials, antimalarial drugs, and...
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    representative, Dr. Daniel Rockey for their support and inspiration. Thanks to the National Institutes of Health

  • Development of Novel Agents to Combat Tuberculosis and Malaria

    Creator:
    Almabruk, Khaled Husain G.
    Abstract:
    Despite the advance of biomedical science, infectious diseases remain one of the number one killers in the world. They claim the lives of millions of people annually, particularly in underdeveloped and developing countries. In fact, more than 32% of the health burden in Africa is caused by infectious diseases, e.g.,...
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    found to have a virulence factor quenching activity in the plant pathogen Rhodococcus fascians. R

  • Biochemical Studies of Pactamycin Biosynthesis in Streptomyces pactum

    Creator:
    Eida, Auday
    Abstract:
    Ribomimetic natural products have long been known for their important biological activities. Structurally, they contain a densely functionalized cyclopentane unit that resembles ribose. Pactamycin, the prominent member of this class of natural products, is a potent protein synthesis inhibitor produced by the soil bacterium Streptomyces pactum. In pactamycin, the aminocyclopentitol...
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    members: Dr. Kerry McPhail, Dr. Benjamin Philmus, Dr. Jane Ishmael, and Dr. Daniel Rocky for their

  • Exploring the cellular mechanisms of Cnidarian bleaching in the sea anemone Aiptasia pallida

    Creator:
    Perez, Santiago
    Abstract:
    Many members of the Phylum Cnidaria are mutualistic with unicellular dinoflagellates belonging to the genus Symbiodinium. Corals are the most widely recognized example of these associations due to their key ecological importance in coral reef ecosystems where they serve as the structural and trophic foundation of these rich ecosystems. Coral...
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    hardships. I thank my committee, Lynda Ciuffetti, Daniel Rockey, Dahong Zhang and Alan Bakalinsky for

  • Characterization of microaggregate formation by Mycobacterium avium subspecies hominissuis

    Creator:
    Babrak, Lmar
    Abstract:
    Mycobacterium avium subsp hominissuis (MAH) is an opportunistic environmental pathogen that causes respiratory and gastrointestinal illness in immunocompromised persons such as those with chronic respiratory diseases or AIDs, respectively. In recent years, there has been a significant increase in the incidence of nontuberculous mycobacterial (NTM) lung infections, including in cystic...
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    ........................ 162 Appendix 1.2 Bannatine JP, Everman JL, Rose SJ, Babrak L Katani R, Barletta RG, Talaat AM

  • Characterization of Mycobacterium avium subsp. hominissuis Interactions with Host Macrophages and Multinucleated Giant Cells

    Creator:
    Joseph, Jayanthi J.
    Abstract:
    To examine the macrophage response to M. avium, I compared inflammasome and cytosolic sensor expression and activation. My result demonstrated that virulent strains of M. avium (A5 and 104) suppress IL-1β production and induce IFN-β production in macrophages. M. avium mutants deficient at DNA export in the biofilm exhibited reduced...
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    , Dr. Elain Fu, Dr. Daniel Rockey, and Dr. Martin Schuster for serving on my graduate committee

  • Infectious diseases of the Felidae : parasite communities from miniature to massive

    Creator:
    Broughton, Heather M.
    Abstract:
    Direct anthropogenic stressors have caused drastic declines in wildlife populations over the past two centuries. In the face of these threats, spillover of infectious disease from domestic animals and livestock into wildlife, and novel interactions between parasites and pathogens within wildlife communities, have further suppressed already vulnerable populations. As management...
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    Software and R (MedCalc Statistical Software bvba, 2014; R Core Team, 14 2014). Prior to

  • Secondary Metabolism in Tolypocladium : Characterization, Ecology, Evolution and Regulation

    Creator:
    Blount, Rheannon Rua
    Abstract:
    An increased understanding of secondary metabolism in fungi is important for both biological and societal reasons. The ascomycete genus, Tolypocladium, is an ideal system to explore secondary metabolism characterization, ecology, evolution and regulation. The genus produces and has the genomic potential to produce a wide array of metabolites. Paired with...
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    helped design, analyze and edit the study. Lanelle R. Connolly provided laboratory support and guidance

  • Further Understanding Mycobacterial Biofilms and Their Interaction with the Host

    Creator:
    Rose, Sasha J.
    Abstract:
    Respiratory infections caused by nontuberculous mycobacteria (NTM), especially Mycobacterium avium, can lead to progressive, recurrent disease that is refractory to therapy. Bacterial biofilms are intrinsically resistant to a variety of stressors and pressures, including host killing mechanisms and antibiotic therapy. Though it is becoming increasingly evident that NTM biofilms are...
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    always been there to give her expertise and advise. I also want to thank Michael Kent, Daniel Rockey

  • Mechanistic Studies and Catalytic Engineering of the Pseudoglycosyltransferase VldE

    Creator:
    Abuelizz, Hatem A.
    Abstract:
    This thesis describes an investigation of the molecular basis of the substrate specificity and catalytic mechanism of a unique pseudoglycosyltransferase (PsGT) enzyme, VldE. PsGTs are a newly discovered group of glycosyltransferase (GT)-like proteins that catalyze the transfer of a pseudosugar to an acceptor molecule via a C-N bond formation. This...
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    Stevens and Dr. Daniel D. Rockey for their invaluable cooperation. I also would like to thank King Saud

  • Surface-exposed proteins in the pathogenesis of Mycobacterium avium subsp. hominissuis

    Creator:
    McNamara, Michael J.
    Abstract:
    Mycobacterium avium subsp. hominissuis (MAH) is a pervasive environmental bacterium that can cause opportunistic infections in humans. Among the most robust and hardy members of the Mycobacterium genus, M. avium can persist and thrive in a range of challenging environments, including many which place it in direct contact with humans....
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    range of un- folded precursor proteins that contain a conserved N-terminal signal sequence (S/T- R-R-X

  • Molecular and cellular characterization of the intracellular phenotype of Mycobacterium avium

    Creator:
    Early, Julie V.
    Abstract:
    Mycobacterium avium cause disseminated disease in immunocompromised people such as AIDS patients. Subsequent to crossing the intestinal epithelium, M. avium thrive within vacuoles in macrophages. The bacteria exhibit a different, more invasive, phenotype after being in macrophages compared to M. avium from laboratory conditions. We hypothesized that this intracellular phenotype...
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    thank my committee members: Daniel Rockey, Nancy Kerkvliet, Ling Jin, and Howard Gelberg for their