The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
Antibacterial activities in the salivary glands of
female mosquitoes Aedes aegypti were investigated in this
study.
The mean salivary bacteriolytic activity, during a
period of 14-day of female mosquitoes exposed to five
different concentrations of Gram-positive bacteria
Micrococcus lysodeikticus in the sucrose meal, was
detected by a lysoplate method. A...
The aim of this study was to gain more information about the
interactions between DNA and the histone octamer during the
process of transcription. This work used a pUC8 plasmid derivative
that contained the core promoter region of the RNA polymerase I of
Acanthamoeba castellanii, placed upstream of four repeats...
Methods are presented to acquire data from analytical ultracentrifugation experiments
by computer using the absorption optical scanning system of the Beckman Model-E
ultracentrifuge. A computer program was written which analyzes sedimentation velocity
experiments by the van Holde - Weischet method and by the second moment method. The van
Holde -...
Agrobacterium tumefaciens is a gram-negative soil bacterium
that causes crown gall tumors on dicotyledenous plants. The
transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing
(Ti) plasmid enters infected plant cells and integrates into
plant nuclear DNA. The T-DNA is accompanied into plant cells by the
VirD2 endonuclease covalently attached...