Six plant pathogenic isolates of Pseudomonas
syringae pv. glycinea, the causal agent of bacterial
blight of Glycine max (L.) Merrill, and one isolate of
P. syringae pv. phaseolicola, the causal agent of halo
blight of Phaseolus vulgaris L., have been examined for
the presence of circular duplex deoxyribonucleic acid
(DNA)....
A sensitive assay for the analysis of contaminating segments
in pseudorecombinants constructed from a seed-transmissible (CVM-Pg)
and a non-seed-transmissible (CMV-Le) strain of cucumber mosaic
virus was developed using partial RNase T₁ digestion. Isolated
genomic RNA segments produced unique digestion patterns when 3'-
end labeled, treated with RNase T₁, and subjected...
The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
Current evidence suggests that temporal regulation of gene
expression in eucaryotic organisms relies primarily upon localized
structural modifications of chromatin. One possible means of
changing chromatin structure is through varying the level of
acetylation of the histone proteins. Sodium butyrate has been found
to inhibit the activity of histone deacetylase...
Antibacterial activities in the salivary glands of
female mosquitoes Aedes aegypti were investigated in this
study.
The mean salivary bacteriolytic activity, during a
period of 14-day of female mosquitoes exposed to five
different concentrations of Gram-positive bacteria
Micrococcus lysodeikticus in the sucrose meal, was
detected by a lysoplate method. A...
The aim of this study was to gain more information about the
interactions between DNA and the histone octamer during the
process of transcription. This work used a pUC8 plasmid derivative
that contained the core promoter region of the RNA polymerase I of
Acanthamoeba castellanii, placed upstream of four repeats...
Methods are presented to acquire data from analytical ultracentrifugation experiments
by computer using the absorption optical scanning system of the Beckman Model-E
ultracentrifuge. A computer program was written which analyzes sedimentation velocity
experiments by the van Holde - Weischet method and by the second moment method. The van
Holde -...