Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a
complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases)
enzymes. One approach to separating these enzymes is affinity chromatography. A new
ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose.
The property of APTC in affinity chromatography is demonstrated using Trichoderma...
Zebrafish (Brachydanio rerio) are small, freshwater teleost fishes in the family
Cyprinidae, the true minnows. They are native to the tropical latitudes of India, but
have become widespread through their use as aquarium fish and as models for several
branches of biological research. Their ease of rearing, short generation time,...
Prostate cancer is the second leading cause of cancer related death in American men. Epidemiologic studies suggest that cruciferous vegetable intake may lower the risk for many cancers, including prostate and colon. Isothiocyanates (ITC) are phytochemicals derived from cruciferous vegetables such as broccoli, Brussels sprouts, cauliflower, and cabbage that may...
Human exposure to the carcinogenic polycyclic aromatic hydrocarbons (PAHs) benzo[a]pyrene (B[a]P) and dibenzo[a,l]pyrene (DB[a,l]P) is through environmental complex mixtures, such as urban dust particulate matter (UDPM) and diesel exhaust (DE), however the potency of these PAH within a mixture is not established. To determine the effect of these mixtures on...
Understanding how wetland birds use habitat is pivotal to developing successful and
beneficial conservation strategies. Although it has been an ardent topic in forest
research for some time, how species interact with the spatial patterning of habitat
across a landscape (i.e., landscape structure) has been more or less neglected in...
The number of drones and genetic diversity among drones are
essential components to a well mated queen. Varroa destructor
preferentially parasitizes drone brood, and is thought to be
responsible for the loss of feral populations that once provided
additional drones for honey bee mating areas. It is necessary to
use...
This dissertation describes studies of isoprenoid biosynthesis in the
phytoflagellate Euglena gracilis. Elucidation of a novel isoprerioid pathway, the 2-Cmethyl-D-erythritol-4-phosphate (MEP) pathway, in addition to the generally known
mevalonate (MVA) pathway, led to extensive exploration of the distribution of both pathways in a variety of organisms. To date, photosynthetic eukaryotes...
Glutathione (GSH) is the predominant low molecular weight thiol antioxidant
in liver tissue. GSH plays an important role in maintaining the intracellular thiol redox
ratio as well as detoxification of electrophiles and xenobiotics. Aging leads to a significant decline (35%; P ≤ 0.05) in hepatocellular GSH levels. Using young (2-4...