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Filtering by: Creator Fike, Jennifer A. Creator Brown, Willie Claiborne Decade 1960-1969

Studies on purification and characterization of an autolytic enzyme from cell walls of Bacillus subtilis

Creator:

Brown, Willie Claiborne

Abstract:

The purpose of this investigation was to purify and characterize the autolytic enzyme from cell walls of Bacillus subtilis 168. The crude enzyme was obtained by autolysis of purified cell walls in buffer at 37° C. Two purification methods were developed. The first involved fractional precipitation with ammonium sulfate. The...

Resource Type:

Dissertation

Full Text:

in the 30 -85% fraction. Purification by this method was 3.2 fold while the recovery was 35 %. A