Index Catalog // ScholarsArchive@OSU

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: VERCHOT AND JAMES C. CARRINGTON* Department of Biology, Texas A&M University, College Station, Texas

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: Functions in trans as an Accessory Factor for Genome Amplification JEANMARIE VERCHOT AND JAMES C

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Creator:: Fahlgren, Noah, Howell, Miya D., Kasschau, Kristin D., Sullivan, Christopher M., Cumbie, Jason S., Givan, Scott A., Law, Theresa F., Grant, Sarah R., Dangl, Jeffrey L., and Carrington, James C.
Abstract:: In plants, microRNAs (miRNAs) comprise one of two classes of small RNAs that function primarily as negative regulators at the posttranscriptional level. Several MIRNA genes in the plant kingdom are ancient, with conservation extending between angiosperms and the mosses, whereas many others are more recently evolved. Here, we use deep...
Resource Type:: Article
Full Text:: . Kasschau1, Elisabeth J. Chapman1,2, Christopher M. Sullivan1, Jason S. Cumbie1, Scott A. Givan1, Theresa F

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Creator:: Carrington, James C. and Freed, Deon D.
Abstract:: The RNA genome of tobacco etch virus (TEV), a plant potyvirus, functions as an mRNA for synthesis of a 346-kilodalton polyprotein that undergoes extensive proteolytic processing. The RNA lacks a normal 5' cap structure at its terminus, which suggests that the mechanism of translational initiation differs from that of a...
Resource Type:: Article
Full Text:: Translation by a Plant Potyvirus 5' Nontranslated Region JAMES C. CARRINGTON* AND DEON D. FREED Department of

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Creator:: Li, Xiao Hua and Carrington, James C.
Abstract:: A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A β-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA amplification. Two mutants (termed VNN...
Resource Type:: Article
Full Text:: transgenic cells XIAo HUA Li AND JAMES C. CARRINGTON* Department of Biology, Texas A&M University, College

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article
Full Text:: Proteinase in Escherichia coli and In Vitro JAMES C. CARRINGTON,* DEON D. FREED, AND TATIA C. SANDERS

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: , and Genome Amplification MARY C. SCHAAD, RUTH HALDEMAN-CAHILL, STEPHEN CRONIN, AND JAMES C

Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon

Creator:: Jeong, Dong-Hoon, Schmidt, Skye A., Rymarquis, Linda A., Park, Sunhee, Ganssmann, Matthias, German, Marcelo A., Accerbi, Monica, Zhai, Jixian, Fahlgren, Noah, Fox, Samuel E., Garvin, David F., Mockler, Todd C., Carrington, James C., Meyers, Blake C., and Green, Pamela J.
Abstract:: BACKGROUND: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation, has been limited in B. distachyon to studies examining a few samples or that rely on computational...
Resource Type:: Article

The Personal Sequence Database: a suite of tools to create and maintain web-accessible sequence databases

Creator:: Givan, Scott A., Sullivan, Christopher M., and Carrington, James C.
Abstract:: Background: Large molecular sequence databases are fundamental resources for modern bioscientists. Whether for project-specific purposes or sharing data with colleagues, it is often advantageous to maintain smaller sequence databases. However, this is usually not an easy task for the average bench scientist. Results: We present the Personal Sequence Database (PSD),...
Resource Type:: Article
Full Text:: databases Scott A Givan*1,2, Christopher M Sullivan1,2 and James C Carrington1,2 Address: 1Center for

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
Resource Type:: Article
Full Text:: . Kasschau, Marilyn Miller, James C. Elizabeth G. Kurth, Valera V. Peremyslov, Alexey I. Interference

ScholarsArchive@OSU

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon

The Personal Sequence Database: a suite of tools to create and maintain web-accessible sequence databases

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

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