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Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

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Carbonell, Alberto, Fahlgren, Noah, Garcia-Ruiz, Hernan, Gilbert, Kerrigan B., Montgomery, Taiowa A., Nguyen, Tammy, Cuperus, Josh T., and Carrington, James C.

Abstract:

In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...

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and decapping in plants (Brodersen et al., 2008) has led to the idea that translation repression could
Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis

Creator:

Lee, Hanna, Yoo, Seong Jeon, Lee, Jeong Hwan, Kim, Wanhui, Yoo, Seung Kwan, Fitzgerald, Heather, Carrington, James C., and Ahn, Ji Hoon

Abstract:

Flowering is the primary trait affected by ambient temperature changes. Plant microRNAs (miRNAs) are small non-coding RNAs playing an important regulatory role in plant development. In this study, to elucidate the mechanism of flowering-time regulation by small RNAs, we identified six ambient temperature-responsive miRNAs (miR156, miR163, miR169, miR172, miR398 and...

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Online, 15, 2730. 9. Fahlgren,N., Howell,M.D., Kasschau,K.D., Chapman,E.J., Sullivan,C.M., Cumbie,J.S

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Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis

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