Index Catalog // ScholarsArchive@OSU

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article
Full Text:: /89/104459-05$02.00/0 Copyright ©) 1989, American Society for Microbiology 4460 NOTES Okb 1 2 3 4

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Creator:: Carrington, James C. and Freed, Deon D.
Abstract:: The RNA genome of tobacco etch virus (TEV), a plant potyvirus, functions as an mRNA for synthesis of a 346-kilodalton polyprotein that undergoes extensive proteolytic processing. The RNA lacks a normal 5' cap structure at its terminus, which suggests that the mechanism of translational initiation differs from that of a...
Resource Type:: Article
Full Text:: /041590-08$02.00/0 Copyright © 1990, American Society for Microbiology Cap-Independent Enhancement of

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: -538X/95/$04.0010 Copyright q 1995, American Society for Microbiology Debilitation of Plant Potyvirus

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: /$04.0010 Copyright q 1995, American Society for Microbiology Evidence that the Potyvirus P1 Proteinase

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: /96/$04.0010 Copyright q 1996, American Society for Microbiology Analysis of the VPg-Proteinase (NIa

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Creator:: Sáenz, Pilar, Salvador, Beatriz, Simón-Mateo, Carmen, Kasschau, Kristin D., Carrington, James C., and García, Juan Antonio
Abstract:: Plum pox virus (PPV) is a member of the Potyvirus genus that, in nature, infects trees of the Prunus genus. Although PPV infects systemically several species of the Nicotiana genus, such as N. clevelandii and N. benthamiana, and replicates in the inoculated leaves of N. tabacum, it is unable to...
Resource Type:: Article
Full Text:: 0022-538X/02/$04.00�0 DOI: 10.1128/JVI.76.4.1922–1931.2002 Copyright © 2002, American Society for

Identification and Characterization of Human Cytomegalovirus-Encoded MicroRNAs

Creator:: Grey, Finn, Antoniewicz, Andy, Allen, Edwards, Saugstad, Julie, McShea, Andy, Carrington, James C., and Nelson, Jay
Abstract:: MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved...
Resource Type:: Article

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Creator:: Cosson, Patrick, Sofer, Luc, Hien Le, Quang, Léger, Valérie, Schurdi-Levraud, Valérie, Whitham, Steven A., Yamamoto, Miki L., Gopalan, Suresh, Le Gall, Olivier, Chandresse, Thierry, Carrington, James C., and Revers, Frédéric
Abstract:: Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins....
Resource Type:: Article
Full Text:: American Society of Plant Biologists Restriction of long-distance movement of several potyviruses

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
Resource Type:: Article

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

Creator:: Carbonell, Alberto, Fahlgren, Noah, Garcia-Ruiz, Hernan, Gilbert, Kerrigan B., Montgomery, Taiowa A., Nguyen, Tammy, Cuperus, Josh T., and Carrington, James C.
Abstract:: In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
Resource Type:: Article

ScholarsArchive@OSU

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Identification and Characterization of Human Cytomegalovirus-Encoded MicroRNAs

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Functional Analysis of Three Arabidopsis ARGONAUTES Using Slicer-Defective Mutants

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