Index Catalog // ScholarsArchive@OSU

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
Resource Type:: Article
Full Text:: endogenous genes via virus-induced gene-silenc- ing (VIGS) technology. Additional advantages of this vector

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Gilbert, Kerrigan B., Takeda, Atsushi, Giampetruzzi, Annalisa, Garcia Ruiz, Mayra T., McGinn, Michaela G., Lowery, Nicholas, Martinez Baladejo, Maria T., and Carrington, James C.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Resource Type:: Article

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Creator:: Cosson, Patrick, Sofer, Luc, Hien Le, Quang, Léger, Valérie, Schurdi-Levraud, Valérie, Whitham, Steven A., Yamamoto, Miki L., Gopalan, Suresh, Le Gall, Olivier, Chandresse, Thierry, Carrington, James C., and Revers, Frédéric
Abstract:: Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins....
Resource Type:: Article
Full Text:: infection sites and is con- trolled by dominant resistance genes (R genes) through a gene-for-gene

Genetic and Functional Diversification of Small RNA Pathways in Plants

Creator:: Xie, Zhixin, Johansen, Lisa K., Gustafan, Adam M., Kasschau, Kristin D., Lellis, Andrew D., Zilberman, Daniel, Jacobsen, Steven E., and Carrington, James C.
Abstract:: Multicellular eukaryotes produce small RNA molecules (approximately 21–24 nucleotides) of two general types, microRNA (miRNA) and short interfering RNA (siRNA). They collectively function as sequence-specific guides to silence or regulate genes, transposons, and viruses and to modify chromatin and genome structure. Formation or activity of small RNAs requires factors belonging...
Resource Type:: Article
Full Text:: * 1 Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming.pdf

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Takeda, Atsushi, Giampetruzzi, Annalisa, Lowery, Nicholas, Carrington, James C., and et al.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
Full Text:: AGO genes have been implicated in antiviral defense: AGO1 against Cucumber mosaic virus (CMV) [25

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming_SupportingInformation.zip

Creator:: Garcia-Ruiz, Hernan, Carbonell, Alberto, Hoyer, J. Steen, Fahlgren, Noah, Takeda, Atsushi, Giampetruzzi, Annalisa, Lowery, Nicholas, Carrington, James C., and et al.
Abstract:: In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Creator:: Sáenz, Pilar, Salvador, Beatriz, Simón-Mateo, Carmen, Kasschau, Kristin D., Carrington, James C., and García, Juan Antonio
Abstract:: Plum pox virus (PPV) is a member of the Potyvirus genus that, in nature, infects trees of the Prunus genus. Although PPV infects systemically several species of the Nicotiana genus, such as N. clevelandii and N. benthamiana, and replicates in the inoculated leaves of N. tabacum, it is unable to...
Resource Type:: Article
Full Text:: mutations in the potyvirus HC-Pro gene affect helper compo- nent activity, virus accumulation, and symptom

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Creator:: Li, Xiao Hua and Carrington, James C.
Abstract:: A genetic complementation system was developed in which tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated with NIb-defective TEV mutants. A β-glucuronidase (GUS) reporter gene integrated into the genomes of parental and four mutant viruses was used to assay RNA amplification. Two mutants (termed VNN...
Resource Type:: Article
Full Text:: tobacco etch virus (TEV) polymerase (NIb)- expressing transgenic plants or protoplasts were inoculated

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
Resource Type:: Article
Full Text:: –240. 38. Jefferson, R. A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

Creator:: Verchot, Jeanmarie and Carrington, James C.
Abstract:: Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
Resource Type:: Article
Full Text:: 77843 Received 14 October 1994/Accepted 23 November 1994 Tobacco etch virus (TEV) encodes three

ScholarsArchive@OSU

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein

Genetic and Functional Diversification of Small RNA Pathways in Plants

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming.pdf

Garcia-RuizHernanBotanyPlantPathologyRolesProgramming_SupportingInformation.zip

Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells

Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

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