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Filtering by: Subject DNA-protein interactions Remove constraint Subject: DNA-protein interactions Creator Chen, Cheng Yao Remove constraint Creator: Chen, Cheng Yao

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  • Biochemical characterization and mutational analysis of human uracil-DNA glycosylase

    Creator:
    Chen, Cheng-Yao
    Abstract:
    PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...
    Resource Type:
    Dissertation
    Full Text:
    R276 mutant proteins formed a stable complex with the uracil-DNA glycosylase inhibitor protein (Ugi