Eukaryotic ribosome biogenesis involves ~200 assembly
factors, but how these contribute to ribosome
maturation is poorly understood. Here, we
identify a network of factors on the nascent 60S subunit
that actively remodels preribosome structure. At its hub is
Rsa4, a direct substrate of the force-generating ATPase
Rea1. We show that...
Eukaryotic ribosome biogenesis involves ~200 assembly
factors, but how these contribute to ribosome
maturation is poorly understood. Here, we
identify a network of factors on the nascent 60S subunit
that actively remodels preribosome structure. At its hub is
Rsa4, a direct substrate of the force-generating ATPase
Rea1. We show that...
Capture-recapture studies offer a powerful tool to assess abundance, survival and population rate of change (λ). A previous capture-recapture study, based on DNA profiles, estimated that the IUCN-listed Endangered Oceania population of humpback whales had a superpopulation size of 4329 whales (95% confidence limits, CL: 3345, 5315) and λ =...
Capture-recapture studies offer a powerful tool to assess abundance, survival and population rate of change (λ). A previous capture-recapture study, based on DNA profiles, estimated that the IUCN-listed Endangered Oceania population of humpback whales had a superpopulation size of 4329 whales (95% confidence limits, CL: 3345, 5315) and λ =...
Hydroacoustic data document the occurrence of 5 flare clusters and several single flares from which bubbles rise through the entire water column from an active seep site at 40 m water depth in the central North Sea. We investigated the difference in dissolved methane distributions along a 6 km transect...
Hydroacoustic data document the occurrence of 5 flare clusters and several single flares from which bubbles rise through the entire water column from an active seep site at 40 m water depth in the central North Sea. We investigated the difference in dissolved methane distributions along a 6 km transect...
We investigated dissolved methane distributions along a 6 km transect crossing active seep sites at 40 m water depth in the central North Sea. These investigations were done under conditions of thermal stratification in summer (July 2013) and homogenous water column in winter (January 2014). Dissolved methane accumulated below the...
We investigated dissolved methane distributions along a 6 km transect crossing active seep sites at 40 m water depth in the central North Sea. These investigations were done under conditions of thermal stratification in summer (July 2013) and homogenous water column in winter (January 2014). Dissolved methane accumulated below the...
Large amount of organic carbon is stored in high latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
Large amount of organic carbon is stored in high latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
A large amount of organic carbon is stored in high-latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
A large amount of organic carbon is stored in high-latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort
among model plant genome databases and plant
researchers that aims to create, maintain and
facilitate the use of a controlled vocabulary
(ontology) for plants. The ontology allows users to
ascribe attributes of plant structure (anatomy and
morphology) and developmental stages...
The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort
among model plant genome databases and plant
researchers that aims to create, maintain and
facilitate the use of a controlled vocabulary
(ontology) for plants. The ontology allows users to
ascribe attributes of plant structure (anatomy and
morphology) and developmental stages...
The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort
among model plant genome databases and plant
researchers that aims to create, maintain and
facilitate the use of a controlled vocabulary
(ontology) for plants. The ontology allows users to
ascribe attributes of plant structure (anatomy and
morphology) and developmental stages...
The farnesoid X receptor (FXR) regulates bile acid, lipid and glucose metabolism. Here we show that treatment of mice with glycine-β-muricholic acid (Gly-MCA) inhibits FXR signalling exclusively in intestine, and improves metabolic parameters in mouse models of obesity. Gly-MCA is a selective high-affinity FXR inhibitor that can be administered orally...
The farnesoid X receptor (FXR) regulates bile acid, lipid and glucose metabolism. Here we show that treatment of mice with glycine-β-muricholic acid (Gly-MCA) inhibits FXR signalling exclusively in intestine, and improves metabolic parameters in mouse models of obesity. Gly-MCA is a selective high-affinity FXR inhibitor that can be administered orally...
To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots
(FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant
species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone)
and compared the rates...
To determine how well DNA barcodes from the chloroplast region perform in forest dynamics plots
(FDPs) from global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant
species from a wide phylogenetic range (3 FDPs in tropics, 5 in subtropics and 5 in temperate zone)
and compared the rates...
Background:
Mapping and map-based cloning of genes that control agriculturally and economically important traits remain great challenges for plants with complex highly repetitive genomes such as those within the grass tribe, Triticeae. Mapping limitations in the Triticeae are primarily due to low frequencies of polymorphic gene markers and poor genetic...
Background:
Mapping and map-based cloning of genes that control agriculturally and economically important traits remain great challenges for plants with complex highly repetitive genomes such as those within the grass tribe, Triticeae. Mapping limitations in the Triticeae are primarily due to low frequencies of polymorphic gene markers and poor genetic...
Resolving the debate surrounding the nature and controls of seasonal variation in the structure and metabolism of Amazonian rainforests is critical to understanding their response to climate change. In situ studies have observed higher photosynthetic and evapotranspiration rates, increased litterfall and leaf flushing during the Sunlight-rich dry season. Satellite data...
Resolving the debate surrounding the nature and controls of seasonal variation in the structure and metabolism of Amazonian rainforests is critical to understanding their response to climate change. In situ studies have observed higher photosynthetic and evapotranspiration rates, increased litterfall and leaf flushing during the Sunlight-rich dry season. Satellite data...
Species distribution models (SDMs) are increasingly applied in conservation management
to predict suitable habitat for poorly known populations. High predictive performance of
SDMs is evident in validations performed within the model calibration area (interpolation),
but few studies have assessed SDM transferability to novel areas (extrapolation), particularly
across large spatial scales...
Species distribution models (SDMs) are increasingly applied in conservation management
to predict suitable habitat for poorly known populations. High predictive performance of
SDMs is evident in validations performed within the model calibration area (interpolation),
but few studies have assessed SDM transferability to novel areas (extrapolation), particularly
across large spatial scales...
BACKGROUND: After 2 decades of focused efforts to eradicate polio, the impact of eradication activities on health
systems continues to be controversial. This study evaluated the impact of polio eradication activities on routine immunization
(RI) and primary healthcare (PHC).
METHODS: Quantitative analysis assessed the effects of polio eradication campaigns on...
BACKGROUND: After 2 decades of focused efforts to eradicate polio, the impact of eradication activities on health
systems continues to be controversial. This study evaluated the impact of polio eradication activities on routine immunization
(RI) and primary healthcare (PHC).
METHODS: Quantitative analysis assessed the effects of polio eradication campaigns on...
Biodiversity continues to decline in the face of increasing anthropogenic pressures such as habitat destruction, exploitation, pollution and introduction of alien species. Existing global databases of species’ threat status or population time series are dominated by charismatic species. The collation of datasets with broad taxonomic and biogeographic extents, and that...
Biodiversity continues to decline in the face of increasing anthropogenic pressures such as habitat destruction, exploitation, pollution and introduction of alien species. Existing global databases of species’ threat status or population time series are dominated by charismatic species. The collation of datasets with broad taxonomic and biogeographic extents, and that...
Biodiversity continues to decline in the face of increasing anthropogenic pressures such as habitat destruction, exploitation, pollution and introduction of alien species. Existing global databases of species’ threat status or population time series are dominated by charismatic species. The collation of datasets with broad taxonomic and biogeographic extents, and that...
Advances in forest carbon mapping have the potential
to greatly reduce uncertainties in the global carbon
budget and to facilitate effective emissions mitigation strategies
such as REDDC (Reducing Emissions from Deforestation
and Forest Degradation). Though broad-scale mapping
is based primarily on remote sensing data, the accuracy of
resulting forest carbon...
Advances in forest carbon mapping have the potential
to greatly reduce uncertainties in the global carbon
budget and to facilitate effective emissions mitigation strategies
such as REDDC (Reducing Emissions from Deforestation
and Forest Degradation). Though broad-scale mapping
is based primarily on remote sensing data, the accuracy of
resulting forest carbon...
Resazurin (Raz) and its reaction product resorufin
(Rru) have increasingly been used as reactive tracers to quantify
metabolic activity and hyporheic exchange in streams.
Previous work has indicated that these compounds undergo
sorption in stream sediments. We present laboratory experiments
on Raz and Rru transport, sorption, and transformation,
consisting of...
Resazurin (Raz) and its reaction product resorufin
(Rru) have increasingly been used as reactive tracers to quantify
metabolic activity and hyporheic exchange in streams.
Previous work has indicated that these compounds undergo
sorption in stream sediments. We present laboratory experiments
on Raz and Rru transport, sorption, and transformation,
consisting of...
Resazurin (Raz) and its reaction product resorufin
(Rru) have increasingly been used as reactive tracers to quantify
metabolic activity and hyporheic exchange in streams.
Previous work has indicated that these compounds undergo
sorption in stream sediments. We present laboratory experiments
on Raz and Rru transport, sorption, and transformation,
consisting of...
Circadian clocks coordinate physiological, neurological, and behavioral functions into circa 24 hour rhythms, and the
molecular mechanisms underlying circadian clock oscillations are conserved from Drosophila to humans. Clock oscillations
and clock-controlled rhythms are known to dampen during aging; additionally, genetic or environmental clock disruption
leads to accelerated aging and increased...
Circadian clocks coordinate physiological, neurological, and behavioral functions into circa 24 hour rhythms, and the
molecular mechanisms underlying circadian clock oscillations are conserved from Drosophila to humans. Clock oscillations
and clock-controlled rhythms are known to dampen during aging; additionally, genetic or environmental clock disruption
leads to accelerated aging and increased...
The goal of this project was to investigate the effects and possible developmental disease implication of chronic dietary
TCDD exposure on global gene expression anchored to histopathologic analysis in juvenile zebrafish by functional genomic,
histopathologic and analytic chemistry methods. Specifically, juvenile zebrafish were fed Biodiet starter with TCDD added at...
The goal of this project was to investigate the effects and possible developmental disease implication of chronic dietary
TCDD exposure on global gene expression anchored to histopathologic analysis in juvenile zebrafish by functional genomic,
histopathologic and analytic chemistry methods. Specifically, juvenile zebrafish were fed Biodiet starter with TCDD added at...
Fungal hyphae are among the most highly polarized cells. Hyphal polarized
growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily
in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex
is required for tethering of secretory vesicles to the apical plasma membrane. We...
Fungal hyphae are among the most highly polarized cells. Hyphal polarized
growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily
in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex
is required for tethering of secretory vesicles to the apical plasma membrane. We...
Eucalypts are the world’s most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have
made them a global renewable resource of fibre and energy. We sequenced and assembled >94% of the 640-megabase
genome of Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, the largest...
Eucalypts are the world’s most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have
made them a global renewable resource of fibre and energy. We sequenced and assembled >94% of the 640-megabase
genome of Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, the largest...
Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may
disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and
non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst
tethering complex, we...
Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may
disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and
non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst
tethering complex, we...
The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the final product BS. Incomplete conversion...
The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the final product BS. Incomplete conversion...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...