Index Catalog // ScholarsArchive@OSU

Unraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca²⁺ Imaging

Creator:: Tang, Longteng, Liu, Weimin, Wang, Yanli, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Imaging Ca²⁺ dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca²⁺-biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca²⁺ binding, a significant population of the chromophores becomes deprotonated. It...
Resource Type:: Article
Full Text:: achieve simultaneously high time resolution (<25 fs) and spectral resolution (<10 cm-1). We exploit FSRS

Excited-State Proton Transfer of Photoexcited Pyranine in Water Observed by Femtosecond Stimulated Raman Spectroscopy

Creator:: Han, Fangyuan, Liu, Weimin, and Fang, Chong
Abstract:: We use femtosecond stimulated Raman spectroscopy (FSRS) to illuminate the choreography of intermolecular excited-state proton transfer (ESPT) of photoacid pyranine (8-hydroxypyrene-1,3,6-trisulfonic acid, HPTS) in water. The multidimensional reaction coordinate responsible for photoacidity is revealed to involve sequential activation of characteristic skeletal motions during the ca. 1 ps preparation stage preceding...
Resource Type:: Article
Full Text:: electronic redistribution and hydrogen bond rearrangement in sub-femtosecond (sub-fs) to fs time range

Cascaded four-wave mixing for broadband tunable laser sideband generation

Creator:: Liu, Weimin, Zhu, Liangdong, Wang, Liang, and Fang, Chong
Abstract:: We demonstrate the versatile broadband wavelength tunability of frequency upconverted multicolor cascaded fourwave-mixing (CFWM) signals spanning the continuous wavelength range from UV to near IR in a thin type-I BBO crystal using 35 fs, 800 nm fundamental and chirped IR supercontinuum white light pulses. Two sets of spatially dispersed CFWM...
Resource Type:: Article
Full Text:: signals at any given moment is between 1000 and 1500 cm−1, corresponding to a pulse duration of 10–15 fs

Uncovering the Hidden Excited State toward Fluorescence of an Intracellular pH Indicator

Creator:: Tang, Longteng, Zhu, Liangdong, Wang, Yanli, and Fang, Chong
Abstract:: Intracellular pH (pH(i)) imaging is of paramount importance for life sciences. In this work, we implement the ultrafast electronic and stimulated Raman spectroscopies to unravel the fluorescence mechanism of an excitation-ratiometric pH(i) indicator in basic aqueous solution. After photoexcitation of the pH(i) indicator HPTS, a hidden charge-transfer (CT) state following...
Resource Type:: Article
Full Text:: formation on the 700 fs time scale. Dynamic correlation with the other excited-state Raman marker bands

A versatile femtosecond stimulated Raman spectroscopy setup with tunable pulses in the visible to near infrared

Creator:: Zhu, Liangdong, Liu, Weimin, and Fang, Chong
Abstract:: We demonstrate a versatile and efficient setup to perform femtosecond stimulated Raman spectroscopy (FSRS). Technical innovations are implemented to achieve the wavelength tunability for both the picosecond narrowband Raman pump pulse and femtosecond broadband Raman probe pulse. Using a simplified one-grating scheme in a home-built second harmonic bandwidth compressor followed...
Resource Type:: Article
Full Text:: , biology, and materials science with the advancement of femtosecond (fs) laser technologies.1–4 Using

Elucidating low-frequency vibrational dynamics in calcite and water with time-resolved third-harmonic generation spectroscopy

Creator:: Wang, Liang, Liu, Weimin, and Fang, Chong
Abstract:: Low-frequency vibrations are foundational for material properties including thermal conductivity and chemical reactivity. To resolve the intrinsic molecular conformational dynamics in condensed phase, we implement time-resolved third-harmonic generation (TRTHG) spectroscopy to unravel collective skeletal motions in calcite, water, and aqueous salt solution in situ. The lifetime of three Raman-active modes...
Resource Type:: Article
Full Text:: +?(/4+I478*L425>(#)4(2*948*:4(F*994/4074(*+(F.4(8-(7/5+8122-G/1I)*7(F49478+(10F(10)1/:-0*7(499478+>( `5

Probing structural evolution along multidimensional reaction coordinates with femtosecond stimulated Raman spectroscopy

Creator:: Frontiera, Renee R., Fang, Chong, Dasgupta, Jyotishman, and Mathies, Richard A.
Abstract:: Mapping out multidimensional potential energy surfaces has been a goal of physical chemistry for decades in the quest to both predict and control chemical reactivity. Recently a new spectroscopic approach called Femtosecond Stimulated Raman Spectroscopy or FSRS was introduced that can structurally interrogate multiple dimensions of a reactive potential energy...
Resource Type:: Article
Full Text:: probe pulse, and can be as short as ~ 10 fs with an appropriate laser system. The first pair of Raman

In-situ weak-beam and polarization control of multidimensional laser sidebands for ultrafast optical switching

Creator:: Liu, Weimin, Wang, Liang, and Fang, Chong
Abstract:: All-optical switching has myriad applications in optoelectronics, optical communications, and quantum information technology. To achieve ultrafast optical switching in a compact yet versatile setup, we demonstrate distinct sets of two-dimensional (2D) broadband up-converted multicolor arrays (BUMAs) in a thin type-I β-barium-borate crystal with two noncollinear near-IR femtosecond pulses at various...
Resource Type:: Article
Full Text:: provides 35 fs, 800 nm pulses at 1 kHz repetition rate [Fig. 1]. Part of the output (�10 lJ/pulse) is

Excited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca²⁺ Imaging Studied by Femtosecond Stimulated Raman Spectroscopy

Creator:: Wang, Yanli, Tang, Longteng, Liu, Weimin, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Resource Type:: Article
Full Text:: with a <250 fs time constant. Upon Ca2+ binding, the chromophore adopts a more twisted conformation

Observation of sum-frequency-generation-induced cascaded four-wave mixing using two crossing femtosecond laser pulses in a 0.1 mm beta-barium-borate crystal

Creator:: Liu, Weimin, Zhu, Liangdong, and Fang, Chong
Abstract:: We demonstrate the simultaneous generation of multicolor femtosecond laser pulses spanning the wavelength range from UV to near IR in a 0.1 mm Type I beta-barium borate crystal from 800 nm fundamental and weak IR super-continuum white light (SCWL) pulses. The multicolor broadband laser pulses observed are attributed to two...
Resource Type:: Article
Full Text:: . Over the past decade, broadband and wavelength- tunable femtosecond (fs) laser pulses from ultraviolet

ScholarsArchive@OSU

Unraveling Ultrafast Photoinduced Proton Transfer Dynamics in a Fluorescent Protein Biosensor for Ca²⁺ Imaging

Excited-State Proton Transfer of Photoexcited Pyranine in Water Observed by Femtosecond Stimulated Raman Spectroscopy

Cascaded four-wave mixing for broadband tunable laser sideband generation

Uncovering the Hidden Excited State toward Fluorescence of an Intracellular pH Indicator

A versatile femtosecond stimulated Raman spectroscopy setup with tunable pulses in the visible to near infrared

Elucidating low-frequency vibrational dynamics in calcite and water with time-resolved third-harmonic generation spectroscopy

Probing structural evolution along multidimensional reaction coordinates with femtosecond stimulated Raman spectroscopy

In-situ weak-beam and polarization control of multidimensional laser sidebands for ultrafast optical switching

Excited State Structural Events of a Dual-Emission Fluorescent Protein Biosensor for Ca²⁺ Imaging Studied by Femtosecond Stimulated Raman Spectroscopy

Observation of sum-frequency-generation-induced cascaded four-wave mixing using two crossing femtosecond laser pulses in a 0.1 mm beta-barium-borate crystal

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