Index Catalog // ScholarsArchive@OSU

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Williams, David E., and Baird, William M.
Abstract:: Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...
Resource Type:: Research Paper
Full Text:: with environmental PAH mixtures Andrew Larkina,b, Lisbeth K. Siddensb, Sharon K. Kruegerc, David E

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

Creator:: Larkin, Andrew, Siddens, Lisbeth K., Krueger, Sharon K., Tilton, Susan C., Waters, Katrina M., Williams, David E., and Baird, William M.
Abstract:: Polycyclic aromatic hydrocarbons (PAHs) are present in the environment as complex mixtures with components that have diverse carcinogenic potencies and mostly unknown interactive effects. Non-additive PAH interactions have been observed in regulation of cytochrome P450 (CYP) gene expression in the CYP1 family. To better understand and predict biological effects of...
Resource Type:: Article
Full Text:: . Waters c,e, David E. Williams a,c,d,f,⁎, William M. Baird a,c,f a Department of Environmental and

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing.pdf

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...
Full Text:: Hydrogen Peroxide Siddens, L. K., Krueger, S. K., Henderson, M. C., & Williams, D. E. (2014). Mammalian

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing_Figures1-9.zip

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...

Mammalian Flavin-Containing Monooxygenase (FMO) as a Source of Hydrogen Peroxide

Creator:: Siddens, Lisbeth K., Krueger, Sharon K., Henderson, Marilyn C., and Williams, David E.
Abstract:: Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and 3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂ upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production. Two common FMO2 (the major...
Resource Type:: Article

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

Creator:: Tilton, Susan C., Siddens, Lisbeth K., Krueger, Sharon K., Larkin, Andrew J., Löhr, Christiane V., Williams, David E., Baird, William M., and Waters, Katrina M.
Abstract:: We have previously shown that relative potency factors and DNA adduct measurements are inadequate for predicting carcinogenicity of certain polycyclic aromatic hydrocarbons (PAHs) and PAH mixtures, particularly those that function through alternate pathways or exhibit greater promotional activity compared to benzo[a]pyrene (BaP). Therefore, we developed a pathway based approach for...
Resource Type:: Article
Full Text:: Christiane V. Löhr § , David E. Williams* ,†,‡ , William M. Baird* ,†, , Katrina M. Waters* ,¶ 4 5

Cytochrome P450 1b1 in polycyclic aromatic hydrocarbon (PAH)-induced skin carcinogenesis: Tumorigenicity of individual PAHs and coal-tar extract, DNA adduction and expression of select genes in the Cyp1b1 knockout mouse

Creator:: Siddens, Lisbeth K., Bunde, Kristi L., Harper, Tod A., McQuistan, Tammie J., Löhr, Christiane V., Bramer, Lisa M., Waters, Katrina M., Tilton, Susan C., Krueger, Sharon K., Williams, David E., and Baird, William M.
Abstract:: FVB/N mice wild-type, heterozygous or null for Cyp 1b1 were used in a two-stage skin tumor study comparing PAH, benzo[a]pyrene (BaP), dibenzo[def,p]chrysene (DBC), and coal tar extract (CTE, SRM 1597a). Following 20 weeks of promotion with TPA the Cyp 1b1 null mice, initiated with DBC, exhibited reductions in incidence, multiplicity,...
Resource Type:: Article
Full Text:: , David E. Williams a,b,c,d,⁎, William M. Baird a,b,d a Department of Environmental and Molecular

Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

Creator:: Siddens, Lisbeth K., Larkin, Andrew, Krueger, Sharon K., Bradfield, Christopher A., Waters, Katrina M., Tilton, Susan C., Pereira, Cliff B., Lohr, Christiane V., Arlt, Volker M., Phillips, David H., Williams, David E., and Baird, William M.
Abstract:: The polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), was compared to dibenzo[def,p]chrysene (DBC) and combinations of three environmental PAH mixtures (coal tar, diesel particulate and cigarette smoke condensate) using a two stage, FVB/N mouse skin tumor model. DBC (4 nmol) was most potent, reaching 100% tumor incidence with a shorter latency...
Resource Type:: Article
Full Text:: i, David E. Williams a,b,c,g,⁎, William M. Baird a,b,g a Department of Environmental and Molecular

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure4A).pdf

Creator:: Henderson, Marilyn C., Siddens, Lisbeth K., Krueger, Sharon K., Stevens, J. Fred, Kedzie, Karen, Fang, Ken, Heidelbaugh, Todd, Phong Nguyen, Chow, Ken, Garst, Michael, Gil, Daniel, and Williams, David E.
Abstract:: Mammalian flavin-containing monooxygenase (FMO) is active towards many drugs with a heteroatom having the properties of a soft nucleophile. Thiocarbamides and thiones are S-oxygenated to the sulfenic acid which can either react with glutathione and initiate a redox-cycle or be oxygenated a second time to the unstable sulfinic acid. In...

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3C).pdf

Creator:: Henderson, Marilyn C., Siddens, Lisbeth K., Krueger, Sharon K., Stevens, J. Fred, Kedzie, Karen, Fang, Ken, Heidelbaugh, Todd, Phong Nguyen, Chow, Ken, Garst, Michael, Gil, Daniel, and Williams, David E.
Abstract:: Mammalian flavin-containing monooxygenase (FMO) is active towards many drugs with a heteroatom having the properties of a soft nucleophile. Thiocarbamides and thiones are S-oxygenated to the sulfenic acid which can either react with glutathione and initiate a redox-cycle or be oxygenated a second time to the unstable sulfinic acid. In...
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ScholarsArchive@OSU

Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

Application of a fuzzy neural network model in predicting polycyclic aromatic hydrocarbon-mediated perturbations of the Cyp1b1 transcriptional regulatory network in mouse skin

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing.pdf

SiddensLisbethEnvMolecularToxicMammalianFlavin-Containing_Figures1-9.zip

Mammalian Flavin-Containing Monooxygenase (FMO) as a Source of Hydrogen Peroxide

Mechanism-based classification of PAH mixtures to predict carcinogenic potential

Cytochrome P450 1b1 in polycyclic aromatic hydrocarbon (PAH)-induced skin carcinogenesis: Tumorigenicity of individual PAHs and coal-tar extract, DNA adduction and expression of select genes in the Cyp1b1 knockout mouse

Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure4A).pdf

KruegerSharonEnviroMolecToxicolFlavinContainingMonooxygenase(Figure3C).pdf

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