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  • Molecular cloning and sequence analysis of cystatin from rainbow trout (Oncorhynchus mykiss)

    Creator:
    Li, Fugen
    Abstract:
    A partial cystatin cDNA from rainbow trout was generated by reverse transcription polymerase chain reaction with two degenerate primers. The partial cystatin PCR product was 168 bp and used to screen trout liver λgt 11 cDNA library. Four positive clones were isolated and designated as cstl, cst2, cst3 and cst4....
    Resource Type:
    Masters Thesis
    Full Text:
    previously. Molecular cloning of this cystatin is useful for studies of biological function and genetic

  • Rainbow trout cystatin C : gene expression, heterologous production and characterization

    Creator:
    Li, Fugen
    Abstract:
    Rainbow trout cystatin C cDNA has been isolated from trout liver. The full-length cystatin cDNA (674 bp) included the 5'untranslated region and the polyadenylation signal sequence AATAAA in the 3' region. Translation of the cDNA defines 132 amino acid residues. Comparison of the amino acid sequence with those of family...
    Resource Type:
    Dissertation
    Full Text:
    Philosophy in Molecular and Cellular Biology presented on July 17, 1998. Title: Rainbow Trout Cystatin