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Secondary Metabolism and Interspecific Competition Affect Accumulation of Spontaneous Mutants in the GacS-GacA Regulatory System in Pseudomonas protegens

Creator:: Yan, Qing, Lopes, Lucas D., Shaffer, Brenda T., Kidarsa, Teresa A., Vining, Oliver, Philmus, Benjamin, Song, Chunxu, Stockwell, Virginia O., Raaijmakers, Jos M., McPhail, Kerry L., Andreote, Fernando D., Chang, Jeff H., and Loper, Joyce E.
Abstract:: Secondary metabolites are synthesized by many microorganisms and provide a fitness benefit in the presence of competitors and predators. Secondary metabolism also can be costly, as it shunts energy and intermediates from primary metabolism. In Pseudomonas spp., secondary metabolism is controlled by the GacS-GacA global regulatory system. Intriguingly, spontaneous mutations...
Resource Type:: Article
Full Text:: purified pyoluteorin at 10 �g/ml compared to the equivalent methanol control. The percentage of Gac

The Gac regulon of Pseudomonas fluorescens SBW25

Creator:: Cheng, Xu, de Bruijn, Irene, van der Voort, Menno, Loper, Joyce E., and Raaijmakers, Jos M.
Abstract:: Transcriptome analysis of Pseudomonas fluorescensSBW25 showed that 702 genes were differentially regulated in a gacS::Tn5 mutant, with 300 and 402 genes up- and downregulated respectively. Similar to the Gac regulon of other Pseudomonas species, genes involved in motility, biofilm formation, siderophore biosynthesis and oxidative stress were differentially regulated in the...
Resource Type:: Article
Full Text:: regulon of Pseudomonas fluorescens SBW25 Xu Cheng,1† Irene de Bruijn,1† Menno van der Voort,1† Joyce E

Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS

Creator:: Kidarsa, Teresa A., Shaffer, Brenda T., Goebel, Neal C., Roberts, Daniel P., Buyer, Jeffrey S., Johnson, Aaron, Kobayashi, Donald Y., Zabriskie, T. Mark, Paulsen, Ian, and Loper, Joyce E.
Abstract:: Gene expression profiles of the biological control strain Pseudomonas protegens Pf-5 inhabiting pea seed surfaces were revealed using a whole-genome oligonucleotide microarray. We identified genes expressed by Pf-5 under the control of two global regulators (GacA and RpoS) known to influence biological control and secondary metabolism. Transcript levels of 897...
Resource Type:: Article
Full Text:: 4 6 PFL_ 0639 2786 4092 4656 4732 5545 5957 PFL_ 0639 2786 4092 4656 4732 5545 5957 lo g 2 ta

Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5

Creator:: Hartney, Sierra L., Mazurier, Sylvie, Girard, Maeva K., Mehnaz, Samina, Davis, Edward W. II, Gross, Harald, Lemanceau, Philippe, and Loper, Joyce E.
Abstract:: The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric...
Resource Type:: Article
Full Text:: B (KMB) (27) or in KMB broth at 27°C. Escherichia coli was grown in LB medium (10 g Bacto tryptone

Comparative Genomics of Plant-Associated Pseudomonas spp.: Insights into Diversity and Inheritance of Traits Involved in Multitrophic Interactions

Creator:: Loper, Joyce E., Hassan, Karl A., Mavrodi, Dmitri V., Davis, Edward W., II, Lim, Chee Kent, Shaffer, Brenda T., Elbourne, Liam D. H., Stockwell, Virginia O., Hartney, Sierra L., Breakwell, Katy, Henkels, Marcella D., Tetu, Sasha G., Rangel, Lorena I., Kidarsa, Teresa A., Wilson, Neil L., van de Mortel, Judith E., Song, Chunxu, Blumhagen, Rachel, Radune, Diana, Hostetler, Jessica B., Brinkac, Lauren M., Durkin, A. Scott, Kluepfel, Daniel A., Wechter, W. Patrick, Anderson, Anne J., Kim, Young Cheol, Pierson, Leland S., III, Pierson, Elizabeth A., Lindow, Steven E., Kobayashi, Donald Y., Raaijmakers, Jos M., Weller, David M., Thomashow, Linda S., Allen, Andrew E., and Paulsen, Ian T.
Abstract:: We provide here a comparative genome analysis of ten strains within the Pseudomonas fluorescens group including seven new genomic sequences. These strains exhibit a diverse spectrum of traits involved in biological control and other multitrophic interactions with plants, microbes, and insects. Multilocus sequence analysis placed the strains in three sub-clades,...
Resource Type:: Article
Full Text:: . Henkels1, Sasha G. Tetu3, Lorena I. Rangel2, Teresa A. Kidarsa1, Neil L. Wilson3, Judith E. van de Mortel5

Comparative Genomics of Plant-Associated Pseudomonas spp.: Insights into Diversity and Inheritance of Traits Involved in Multitrophic Interactions

Creator:: Loper, Joyce E., Hassan, Karl A., Mavrodi, Dmitri V., Davis, Edward W., II, Lim, Chee Kent, Shaffer, Brenda T., Elbourne, Liam D. H., Stockwell, Virginia O., Hartney, Sierra L., Breakwell, Katy, Henkels, Marcella D., Tetu, Sasha G., Rangel, Lorena I., Kidarsa, Teresa A., Wilson, Neil L., van de Mortel, Judith E., Song, Chunxu, Blumhagen, Rachel, Radune, Diana, Hostetler, Jessica B., Brinkac, Lauren M., Durkin, A. Scott, Kluepfel, Daniel A., Wechter, W. Patrick, Anderson, Anne J., Kim, Young Cheol, Pierson, Leland S., III, Pierson, Elizabeth A., Lindow, Steven E., Kobayashi, Donald Y., Raaijmakers, Jos M., Weller, David M., Thomashow, Linda S., Allen, Andrew E., and Paulsen, Ian T.
Abstract:: We provide here a comparative genome analysis of ten strains within the Pseudomonas fluorescens group including seven new genomic sequences. These strains exhibit a diverse spectrum of traits involved in biological control and other multitrophic interactions with plants, microbes, and insects. Multilocus sequence analysis placed the strains in three sub-clades,...
Resource Type:: Article
Full Text:: . Henkels1, Sasha G. Tetu3, Lorena I. Rangel2, Teresa A. Kidarsa1, Neil L. Wilson3, Judith E. van de Mortel5

The Effect of Iron Limitation on the Transcriptome and Proteome of Pseudomonas fluorescens Pf-5

Creator:: Lim, Chee Kent, Hassan, Karl A., Tetu, Sasha G., Loper, Joyce E., and Paulsen, Ian T.
Abstract:: One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by...
Resource Type:: Article
Full Text:: Transcriptome and Proteome of Pseudomonas fluorescens Pf-5 Chee Kent Lim1, Karl A. Hassan1, Sasha G. Tetu1

CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf

Creator:: Creason, Allison L., Vandeputte, Olivier M., Savory, Elizabeth A., Davis, Edward W., II, Putnam, Melodie L., Hu, Erdong, Swader-Hines, David, Mol, Adeline, Baucher, Marie, Prinsen, Els, Zdanowska, Magdalena, Givan, Scott A., El Jaziri, Mondher, Loper, Joyce E., Mahmud, Taifo, and Chang, Jeff H.
Abstract:: Members of Gram-positive Actinobacteria cause economically important diseases to plants. Within the Rhodococcus genus, some members can cause growth deformities and persist as pathogens on a wide range of host plants. The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with...
Resource Type:: Article
Full Text:: Fonds David et Alice Van Buuren (Belgium). MB is a Senior Research Associate of the FRS-FNRS (Fonds de

QuecineInterspeciesSignalingSystem.pdf

Creator:: Quecine, Maria Carolina, Kidarsa, Teresa A., Goebel, Neal C., Shaffer, Brenda T., Henkels, Marcella D., Zabriskie, T. Mark, and Loper, Joyce E.
Abstract:: Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A,...
Full Text:: g Difco nutrient broth and 5 g Bacto yeast extract) supplemented with 1% glycerol (NYBGly) (47

ScholarsArchive@OSU

Secondary Metabolism and Interspecific Competition Affect Accumulation of Spontaneous Mutants in the GacS-GacA Regulatory System in Pseudomonas protegens

The Gac regulon of Pseudomonas fluorescens SBW25

Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS

Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5

Comparative Genomics of Plant-Associated Pseudomonas spp.: Insights into Diversity and Inheritance of Traits Involved in Multitrophic Interactions

Comparative Genomics of Plant-Associated Pseudomonas spp.: Insights into Diversity and Inheritance of Traits Involved in Multitrophic Interactions

The Effect of Iron Limitation on the Transcriptome and Proteome of Pseudomonas fluorescens Pf-5

CreasonAllisonBotanyPlantPathologyAnalysisGenomeSequences.pdf

QuecineInterspeciesSignalingSystem.pdf

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