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Filtering by: Advisor Mehl, Ryan Remove constraint Advisor: Mehl, Ryan Creator Machovina, Melodie M. Remove constraint Creator: Machovina, Melodie M.

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  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster

  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster