Because Ras signaling is frequently activated by major hepatocellular carcinoma etiological factors, a transgenic zebrafish constitutively expressing the kras(V12) oncogene in the liver was previously generated by our laboratory. Although this model depicted and uncovered the conservation between zebrafish and human liver tumorigenesis, the low tumor incidence and early mortality...
Here we report a new transgenic expression system by combination of liver-specific expression,
mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic
kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure
of mifepristone to induce permanent genomic recombination mediated by...
Full Text:
expression in transgenic zebrafish
AnhTuan Nguyen
1, 2
, Vivien Koh
1
, Jan M. Spitsbergen
2
Here we report a new transgenic expression system by combination of liver-specific expression,
mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic
kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure
of mifepristone to induce permanent genomic recombination mediated by...
Full Text:
control oncogenic krasV12
expression in transgenic zebrafish
AnhTuan Nguyen1,2, Vivien Koh1, Jan M
Here we report a new transgenic expression system by combination of liver-specific expression, mifepristone induction and Cre-loxP recombination to conditionally control the expression of oncogenic kras[superscript]V12. This transgenic system allowed expression of kras[superscript]V12 specifically in the liver by a brief exposure of mifepristone to induce permanent genomic recombination mediated by...
Human liver cancer is one of the deadliest cancers worldwide, with hepatocellular carcinoma (HCC) being the most common type. Aberrant Ras signaling has been implicated in the development and progression of human HCC, but a complete understanding of the molecular mechanisms of this protein in hepatocarcinogenesis remains elusive. In this...