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Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil

Creator:

Yan, Guiping, Smiley, Richard W., Okubara, Patricia A., Skantar, Andrea M., and Reardon, Catherine L.

Abstract:

Pratylenchus neglectus is one of the most widespread and economically important nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest. It is challenging to quantify P neglectus using microscopic methods for studies that require large-scale sampling, such as assessment of rotation crops, wheat cultivars, and other...

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A. Skantar. b Non-plant-parasitic (Non-PP) communities 1 and 2 were extracted from soils that were
Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae

Creator:

Yan, Guiping, Smiley, Richard W., Okubara, Patricia A., and Skantar, Andrea M.

Abstract:

Heterodera avenae and H. filipjevi are economically important cyst nematodes that restrict production of cereal crops in the Pacific Northwest United States and elsewhere in the world. Identification of these two species is critical for recommending and implementing effective management practices. Primers were designed from the internal transcribed spacer (ITS)...

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stability value predicted to have no amplification. b Name of isolates or clones obtained from GenBank; N