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Creator:: Kandel, Shyam L., Smiley, Richard W., Garland-Campbell, Kimberly, Elling, Axel A., Abatzoglou, John T., Huggins, David, Rupp, Richard, and Paulitz, Timothy C.
Abstract:: Field surveys were conducted by collecting soil samples to estimate nematode densities in soil from winter wheat, spring wheat, spring barley, and spring legumes (lentil, chickpea, and pea) fields during 2010 and 2011. Pratylenchus spp. were observed in 60% of sampled fields. However, nematodes were detected in nearly all of...
Resource Type:: Article
Full Text:: ., and Paulitz, T. C. 2013. Relationship between climatic factors and distribution of Pratylenchus spp

Creator:: Smiley, Richard W. and Marshall, Juliet M.
Abstract:: The cereal cyst nematode Heterodera avenae reduces wheat yield in the Pacific Northwest. Resistance and tolerance traits among spring wheat cultivars were poorly defined. Screening trials were conducted with 39 cultivars over a 2-yr period in irrigated commercial fields that were infested by H. avenae. Comparisons were made between drill...
Resource Type:: Article
Full Text:: into soils that had seed-zone temperatures of 8.9°C in 2013 (4.0-cm depth) and 6.7°C in 2014 (2.4

Creator:: Marshall, Juliet M. and Smiley, Richard W.
Abstract:: Heterodera avenae is a cereal cyst nematode that reduces wheat yields in the Pacific Northwest USA. Barley is also susceptible but there were no previous reports of resistance or tolerance to H. avenae in the USA. Spring barley cultivars were assayed in H. avenae-infested fields over two years. Cultivars were...
Resource Type:: Article
Full Text:: using 0°C as the base temperature at which no heat accumulation or plant growth is calculated. We used

Creator:: Yan, Guiping, Smiley, Richard W., Okubara, Patricia A., Skantar, Andrea M., and Reardon, Catherine L.
Abstract:: Pratylenchus neglectus is one of the most widespread and economically important nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest. It is challenging to quantify P neglectus using microscopic methods for studies that require large-scale sampling, such as assessment of rotation crops, wheat cultivars, and other...
Resource Type:: Article
Full Text:: ., Skantar, A. M., and Reardon, C. L. 2013. Developing a real-time PCR assay for detection and quantifica

Creator:: Yan, Guiping, Smiley, Richard W., and Okubara, Patricia A.
Abstract:: The root-lesion nematode Pratylenchus (hornet is one of the most important pests restricting productivity of wheat in the Pacific Northwest (PNW). It is laborious and difficult to use microscopy to count and identify the nematodes in soils. A SYBR Green I-based real-time polymerase chain reaction (PCR) assay was developed to...
Resource Type:: Article
Full Text:: placed onto sterilized carrot disks to establish pure cultures. Carrot cultures were incu- bated at 22°C

Creator:: Smiley, Richard W. and Yan, Guiping
Abstract:: The cereal cyst nematode Heterodera avenae suppresses wheat production in the western United States. A second species of cereal cyst nematode, H. filipjevi, was identified in eastern Oregon during 2008. This paper reports the discovery of H. filipjevi–infested fields in eastern Washington, thereby extending the known distribution of H. filipjevi...
Resource Type:: Article
Full Text:: Taq polymerase (Roche). PCR amplification was performed as follows; 94°C for 2 min, followed by 40

Creator:: Yan, Guiping, Smiley, Richard W., Okubara, Patricia A., and Skantar, Andrea M.
Abstract:: Heterodera avenae and H. filipjevi are economically important cyst nematodes that restrict production of cereal crops in the Pacific Northwest United States and elsewhere in the world. Identification of these two species is critical for recommending and implementing effective management practices. Primers were designed from the internal transcribed spacer (ITS)...
Resource Type:: Article
Full Text:: , transferred to an Eppendorf tube containing sterilized water, and stored at 4°C for nematode identification

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