Index Catalog // ScholarsArchive@OSU

Creator:: Tang, Longteng, Zhu, Liangdong, Wang, Yanli, and Fang, Chong
Abstract:: Intracellular pH (pH(i)) imaging is of paramount importance for life sciences. In this work, we implement the ultrafast electronic and stimulated Raman spectroscopies to unravel the fluorescence mechanism of an excitation-ratiometric pH(i) indicator in basic aqueous solution. After photoexcitation of the pH(i) indicator HPTS, a hidden charge-transfer (CT) state following...
Resource Type:: Article
Full Text:: Indicator Longteng Tang, Liangdong Zhu, Yanli Wang, and Chong Fang* Department of Chemistry

Creator:: Tachibana, Sean R., Tang, Longteng, Zhu, Liangdong, Liu, Weimin, Wang, Yanli, and Fang, Chong
Abstract:: Biosensors have become an indispensable tool set in life sciences. Among them, fluorescent protein-based biosensors have great biocompatibility and tunable emission properties but their development is largely on trial and error. To facilitate a rational design, we implement tunable femtosecond stimulated Raman spectroscopy, aided by transient absorption and quantum calculations,...
Resource Type:: Article
Full Text:: ,§ Yanli Wang, and Chong Fang* Department of Chemistry, Oregon State University, Corvallis, Oregon

Creator:: Tang, Longteng, Zhu, Liangdong, Taylor, Miles A., Wang, Yanli, Remington, S. James, and Fang, Chong
Abstract:: Tracking vibrational motions during a photochemical or photophysical process has gained momentum, due to its sensitivity to the progression of reaction and change of environment. In this work, we implemented an advanced ultrafast vibrational technique, femtosecond-stimulated Raman spectroscopy (FSRS), to monitor the excited state structural evolution of an engineered green...
Resource Type:: Article
Full Text:: Longteng Tang 1 ID , Liangdong Zhu 1 ID , Miles A. Taylor 1, Yanli Wang 1, S. James Remington 2 and Chong

Creator:: Tang, Longteng, Liu, Weimin, Wang, Yanli, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Imaging Ca²⁺ dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca²⁺-biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca²⁺ binding, a significant population of the chromophores becomes deprotonated. It...
Resource Type:: Article
Full Text:: ] Weimin Liu,[a] Yanli Wang,[a] Yongxin Zhao,[b] Breland G. Oscar, [a] Robert E. Campbell,[b] and Chong

Creator:: Wang, Yanli, Tang, Longteng, Liu, Weimin, Zhao, Yongxin, Oscar, Breland G., Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Resource Type:: Article
Full Text:: Studied by Femtosecond Stimulated Raman Spectroscopy Yanli Wang,a Longteng Tang,a Weimin Liu,a

Creator:: Oscar, Breland G., Liu, Weimin, Zhao, Yongxin, Tang, Longteng, Wang, Yanli, Campbell, Robert E., and Fang, Chong
Abstract:: Fluorescent proteins (FPs) have played a pivotal role in bioimaging and advancing biomedicine. The versatile fluorescence from engineered, genetically encodable FP variants greatly enhances cellular imaging capabilities, which are dictated by excited state structural dynamics of the embedded chromophore inside the protein pocket. Visualization of the molecular choreography of the...
Resource Type:: Article
Full Text:: Tanga, Yanli Wanga, Robert E. Campbellb, and Chong Fanga,2 aDepartment of Chemistry, Oregon State

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