BACKGROUND: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and
biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation,
has been limited in B. distachyon to studies examining a few samples or that rely on computational...
BACKGROUND: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and
biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation,
has been limited in B. distachyon to studies examining a few samples or that rely on computational...
BACKGROUND: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and
biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation,
has been limited in B. distachyon to studies examining a few samples or that rely on computational...
In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short
interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target
loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing
response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short
interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target
loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing
response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has...
BACKGROUND: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and
biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation,
has been limited in B. distachyon to studies examining a few samples or that rely on computational...
In eukaryotes, RNA silencing pathways utilize 20-30-nucleotide small RNAs to regulate gene expression, specify and maintain chromatin structure, and repress viruses and mobile genetic elements. RNA silencing was likely present in the common ancestor of modern eukaryotes, but most research has focused on plant and animal RNA silencing systems. Phytophthora...
The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicer-independent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were...
