The economically viable production of value added fuels and chemicals from lignocellulosic feedstocks hinges on our ability to quickly and efficiently transform structural carbon molecules to end products. The sugars in lignocellulosic biomass are held primarily within cellulose and hemicellulose. Cellulose is composed primarily of glucose monomers which are quickly...
A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further...
A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further...
A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further...
Poly-3-hydroxybutyrate (PHB) is a renewable, biodegradable biopolymer that has
shown great promise to offset the use of hydrocarbon-derived plastics. The genes
encoding the bacterial PHB production pathway have been engineered into several
higher order plant species providing an opportunity to produce PHB as a
co-product on an industrial, agricultural scale....
The production of fuel ethanol from lignocellulosic biomass has the potential to replace a significant portion of non-renewable transport fuels. Woody feedstocks are composed of cellulose, hemicellulose, and lignin. Glucose, the monomer of cellulose, is readily utilized by wild-type S. cerevisiae, but xylose, which comprises 60% of the sugar in...