Index Catalog // ScholarsArchive@OSU

Creator:: Minato, Yusuke, Fassio, Sara R., Kirkwood, Jay S., Halang, Petra, Quinn, Matthew J., Faulkner, Wyatt J., Aagesen, Alisha M., Steuber, Julia, Stevens, Jan F., and Häse, Claudia C.
Abstract:: The Na⁺ translocating NADH:quinone oxidoreductase (Na⁺-NQR) is a unique respiratory enzyme catalyzing the electron transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp., including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Thus, Na⁺-NQR should significantly...
Resource Type:: Article

Creator:: Minato, Yusuke, Fassio, Sara R., Reddekopp, Rylan L., and Häse, Claudia C.
Abstract:: Two virulence factors produced by Vibrio cholerae, cholera toxin (CT) and toxin-corregulated pilus (TCP), are indispensable for cholera infection. ToxT is the central regulatory protein involved in activation of CT and TCP expression. We previously reported that lack of a respiration-linked sodium-translocating NADH–ubiquinone oxidoreductase (Na⁺-NQR) significantly increases toxT transcription. In...
Resource Type:: Article

Creator:: Minato, Yusuke, Ghosh, Amit, Faulkner, Wyatt J., Lind, Erin J., Schesser Bartra, Sara, Plano, Gregory V., Jarrett, Clayton O., Hinnebusch, B. Joseph, Winogrodzki, Judith, Dibrov, Pavel, and Hase, Claudia C.
Abstract:: Na⁺/H⁺ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na⁺/H⁺ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na⁺/H⁺ antiporters, NhaA and NhaB, are completely attenuated in...
Resource Type:: Article

Creator:: Minato, Yusuke, Fassio, Sara R., and Häse, Claudia C.
Abstract:: We previously found that inhibition of the TCA cycle, either through mutations or chemical inhibition, increased toxT transcription in Vibrio cholerae. In this study, we found that the addition of malonate, an inhibitor of succinate dehydrogenase (SDH), decreased toxT transcription in V. cholerae, an observation inconsistent with the previous pattern...
Resource Type:: Article

Creator:: Faulkner, Wyatt J., Ghosh, Amit, Minato, Yusuke, Winogrodzki, Judith, Lind, Erin, Plano, Gregory V., Hinnebusch, Joseph B., Dibrov, Pavel, and Häse, Claudia C.
Abstract:: We found that a strains of Yersinia pestis (KIM5) which lacked the nhaA gene was fully attenuated in a plague model. This gene produces a protein of the sodium-proton antiporter family which expel sodium ions from the bacterial cytoplasm in exchange for hydrogen ions, or protons, from the surrounding environment....
Resource Type:: Poster

Creator:: Fassio, Sara R., Minato, Yusuke, Quinn, Matthew J., Faulkner, Wyatt J., and Hase, Claudia C.
Abstract:: We previously reported that inhibition of the Na⁺ translocating NADH:ubiquinone oxidoreductase (NQR), either by chemical inhibition or mutation, increased toxT transcription in Vibrio cholerae. In this study, we revealed that the nqr mutant strain showed similar phenotypes as the Escherichia coli NADH dehydrogenase I (nuo) mutant strain (e.g. growth defect...
Resource Type:: Poster

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