In order to determine the total number of cytochrome P-450 isozymes
present in a given species following various treatments, mature
male rats (Sprague-Dawley) were injected intraperitoneally with the
classic mixed-function oxidase inducers phenobarbital (PB), pregnenolone-
16a-carbonitrile (PCN), 3-methylcholanthrene (3-MC), or the appropriate
control vehicle.
Hepatic microsomes were solubilized and P-450...