Partial purification of diamine oxidase from pea seedlings,
Pisum sativum, was accomplished by homogenization of 8-10 day
etiolated epicotyl tissue, followed by ammonium sulfate fractionation
and DEAE Sephadex column chromatography. The preparation thus
obtained was purified 50 fold.
Some properties of this enzyme were investigated. A radio-tracer method was adapted...