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Filtering by: Creator Yarwood, R. R. Peer Reviewed Yes

Noninvasive Quantitative Measurement of Bacterial Growth in Porous Media under Unsaturated-Flow Conditions

Creator:

Yarwood, R. R., Rockhold, M. L., Niemet, M. R., Selker, J. S., and Bottomley, P. J.

Abstract:

Glucose-dependent growth of the luxCDABE reporter bacterium Pseudomonas fluorescens HK44 was monitored noninvasively in quartz sand under unsaturated-flow conditions within a 45- by 56- by 1-cm two-dimensional light transmission chamber. The spatial and temporal development of growth were mapped daily over 7 days by quantifying salicylate-induced bioluminescence. A nonlinear model...

Resource Type:

Article
Impact of microbial growth on water flow and solute transport in unsaturated porous media

Creator:

Yarwood, R. R., Rockhold, M. L., Niemet, M. R., Selker, J. S., and Bottomley, P. J.

Abstract:

A novel analytical method was developed that permitted real-time, noninvasive measurements of microbial growth and associated changes in hydrodynamic properties in porous media under unsaturated flowing conditions. Salicylate-induced, lux gene-based bioluminescence was used to quantify the temporal and spatial development of colonization over a 7-day time course. Water contents were...

Resource Type:

Article