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Filtering by: Has journal Plant, Cell & Environment Remove constraint Has journal: Plant, Cell & Environment Decade 1960-1969 Remove constraint Decade: 1960-1969 Degree Field Microbiology Remove constraint Degree Field: Microbiology

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  • Methods for the in vitro cultivation of cells from the tissues of salmonid fishes

    Creator:
    Fryer, John L.
    Abstract:
    Methods examined for the preparation of cells from tissues of salmonid fishes for primary cultivation were the fragment (explant) and enzyme dispersion techniques. Both methods were employed for the cultivation of a variety of tissues from Pacific salmon, Oncorhynchus spp., and the steelhead trout, Salmo gairdneri gairdneri. A technique was...
    Resource Type:
    Dissertation
    Full Text:
    of inoculum were of major concern with regard to the proper environment for cell growth

  • Distribution of Clostridium botulinum type E in fish, shellfish and the marine environment of the Pacific Northwest, and protein patterns of the toxigenic and non-toxigenic stains

    Creator:
    Craig, James Morrison
    Abstract:
    Interest in the distribution of Clostridium botulinum type E was heightened by the sudden outbreak of human botulism from smoked whitefish chubs and canned tuna fish in 1963. The question arose as to how widely the organism is distributed among fish and shellfish in the Northwest and what potential hazard...
    Resource Type:
    Dissertation
    Full Text:
    CLOSTRIDIUM BOTULINUM TYPE E IN FISH SHELLFISH AND THE MARINE ENVIRONMENT OF THE PACIFIC NORTHWEST, AND

  • The lipids of spore types B, E, and F and vegetative cell types E and F of Clostridium botulinum

    Creator:
    Kimble, Charles E.
    Abstract:
    The primary purpose of this investigation was to characterize the lipids of the spores and vegetative cells of Cl. botulinum. A second purpose was to explore the possibility that lipids might serve as a means of differentiating the chiefly proteolytic Cl. botulinum type B from the nonproteolytic Cl. botulinum types...
    Resource Type:
    Masters Thesis
    Full Text:
    The Lipids of Spore Types B, E, and F and Vegetative Cell

  • Ecological study of aquatic myxobacteria in a woodland stream

    Creator:
    Carlson, René Virginia
    Abstract:
    Most studies of bacteria in water have concerned public health; little attention has been given to organisms which are indigenous to the aquatic environment. Myxobacteria are known to occur in soil, in the marine environment, and several have been studied in relation to diseases of salmonid fishes. However, little information...
    Resource Type:
    Masters Thesis
    Full Text:
    indigenous to the aquatic environment. Myxobacteria are known to occur in soil, in the marine environment

  • Properties of Pseudomonas aeruginosa resistant to quaternary ammonium compound

    Creator:
    Washam, Clinton Jay
    Abstract:
    Pseudomonas aeruginosa cells were selected for their ability to grow in the presence of 750 ppm alkyldimethylethylbenzyl ammonium chloride (QAC). These cells were found to retain their resistance to the germicide throughout tri-weekly transfers for 7 months in tryptone glucose yeast extract (TGY) broth containing no QAC. Comparisons of the...
    Resource Type:
    Dissertation
    Full Text:
    . The greatest difference between the two cell types occurred from pH 3. 5 to pH 4. 5 with a second

  • Significance of pigments in radiation resistance of Micrococcus radiodurans

    Creator:
    Krabbenhoft, Kenneth Louis
    Abstract:
    An ecological study of a microorganism similar to that isolated in 1956 and designated as Micrococcus radiodurans indicated that the organism could be isolated from several different environments. By taking advantage of its radio-resistivity, high levels of gamma radiation were used in the isolation procedures to inactivate most of the...
    Resource Type:
    Dissertation
    Full Text:
    plant roots (95), mammalian skin (7, p. 414 -417) and isolated mitochondria (71). Within the cell

  • Fresh water myxobacteria : a taxonomic study

    Creator:
    Nitsos, Peggy Koerner
    Abstract:
    Myxobacteria are known to occur in the aquatic environment, however, little information is available regarding the taxonomy of these organisms. This investigation was initiated in an attempt to classify a group of fresh water cytophagas on the basis of biochemical and physiological as well as cultural and morphological characteristics. The...
    Resource Type:
    Masters Thesis
    Full Text:
    Abstract approved: "Dr. A. E. Pacha Myxobacteria are known to occur in the aquatic environment, however

  • Characterization of the Oregon sockeye salmon virus

    Creator:
    Wingfield, William Harry
    Abstract:
    Characterization of a virus requires that its physical, biochemical and biological properties be determined. In this study the Oregon sockeye salmon virus was characterized. It was isolated in the fall of 1958 from juvenile sockeye salmon, Oncorhynchus nerka, being reared at the Oregon Fish Commission's Willamette River Hatchery. When inoculated...
    Resource Type:
    Dissertation
    Full Text:
    gairdenerli gairdnerii embryonic cells did not become infected upon exposure to this virus. Cell cultures

  • A physiological approach to a rapid method for identifying C. botulinum

    Creator:
    Dyer, John Kaye
    Abstract:
    Disc gel electrophoresis of the vegatative cell -free extracts ofstrains of Clostridium botulinum types A, B, C, E, and F and therelated nontoxic group showed limited value as a means for identifyingthese closely related microorganisms, since separation,though not consistent in all cases, could only be based on the numberof protein...
    Resource Type:
    Dissertation
    Full Text:
    Disc gel electrophoresis of the vegatative cell -free extracts of strains of Clostridium botulinum

  • Excretion of viable salmonellae by Salmonella-fed, chlorine-treated nematodes

    Creator:
    Smerda, Susan Mae
    Abstract:
    The role of nematodes (Diplogaster lheritieri Maupas 1919) as excretors of pathogenic organisms was studied. Salmonella typhi and Salmonella wichita were fed to nematodes for two days. The nematodes were surface sterilized with free chlorine. The activity of the chlorine was stopped by the addition of sodium thiosulfate followed by...
    Resource Type:
    Masters Thesis
    Full Text:
    with decomposing or rotting plant tissues in contact with the soil, or any site where bacteria are