Comparatively little work has been done to determine the ecology
of Clostridium botulinum type E since its initial isolation in the
nineteen-thirties. This spore forming, anaerobic microorganism is
relatively heat labile and has been missed in ecological surveys in
which heat was used to selectively screen for spore formers. Use...
Polyacrylamide gel disc electrophoresis of concentrated culture
media which had supported growth of Clostridium botulinum
types A and B was shown to be effective in separating the toxin
molecules from hemagglutinating molecules. Since the hemagglutinins
of the two types are immunologically identical and are present
in toxin and toxoid preparations,...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...