Experiments were performed to determine whether a direct
correlation exists between the growth rates of tall and dwarf peas
grown either in the light or in the dark and endogenous gibberellin
(GA) content. Three separate but related experimental approaches
were utilized: (1) the determination of possible qualitative and /or
quantitative...
Chemical and biological evaluations were conducted to provide
chemical composition and digestibility information, in addition to
yield data, for silage corn varieties produced on poorly drained
Dayton soil series. Each variety was subjected to a Kjeldahl nitrogen,
acid detergent fiber, acid detergent lignin, cell wall, and
residual ash test. Subsequently...
The behavior of all of the observed molecular band
emission as well as the prominent atomic line emission
from a pulsed microwave helium plasma is given. Extensive
efforts to purify the helium include the use of a
uranium getter, a quartz diffusion tube, and a cataphoretic
discharge. The plasma is...
Routine studies of bacterial fatty acid and lipid composition,
made possible by recently developed techniques, have brought about
an increasing interest in the physiological significance of these
compounds. The purpose of this study was to characterize the fatty
acids of the vegetative cells of Clostridium botulinum 33A, as the
first...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...
Comparatively little work has been done on bacterial lipids.
There are profound differences between bacterial lipids in general
and lipids of the higher forms of life in such respects as the absence
of sterols, phospholipids low in nitrogen but high in inositol
and carbohydrate, the presence of large proportions of...
The primary purpose of this investigation was to characterize
the lipids of the spores and vegetative cells of Cl. botulinum. A
second purpose was to explore the possibility that lipids might serve
as a means of differentiating the chiefly proteolytic Cl. botulinum
type B from the nonproteolytic Cl. botulinum types...
The purpose of this study was to compare and test the efficiency of the leading kinds of media being used for the isolation and identification
of Clostridium botulinum type E strains, and to develop a
more reliable method and media for the differentiation of this organism
based on the "pearly...
Gamma irradiation inactivation kinetics were studied using
fifteen organisms naturally contaminating seafoods. Survival curves
for seven Salmonella, three Shigella, two Streptococcus species and
a Staphylococcus, Escherichia and Proteus species were determined
using Hartsell's broth and fresh oysters. Also, the Shigella species
and one species of Streptococcus, Escherichia and Proteus were...
Studies were conducted under controlled conditions in order
to observe the resulting survival patterns in solid crabmeat of
Salmonella typhosa, Salmonella paratyphi B, and SaImonella wichita
following individual exposure to various doses of gamma radiation.
As shown in their survival curves, these species of Salmonella
exhibited in varying degrees a...
Disc gel electrophoresis of the vegatative cell -free extracts ofstrains of Clostridium botulinum types A, B, C, E, and F and therelated nontoxic group showed limited value as a means for identifyingthese closely related microorganisms, since separation,though not consistent in all cases, could only be based on the numberof protein...
The purpose of this study was to examine the culture
supernatants of various strains of Clostridium botulinum
for characteristic extracellular enzymes which can facilitate
their rapid identification.
A convenient, efficient, water-cooled flat gel electrophoretic
unit was utilized to concentrate and purify the
total proteins and extracellular enzymes present in the...
Studies have been made to determine the limits of tolerance to
gamma radiation for 15 cultures of the following bacterial species
both in culture media and in irradiation-sterilized seafoods (crab-meat,
halibut and shrimp) inoculated with Salmonella (seven species),
Shigella (three species), and one species each of Neisseria, Myco-bacterium, Esctrerichia, Proteus...
Numerous attempts have been made to identify the flavor
compounds in Blue cheese, however, duplication of Blue cheese
flavor has not yet been accomplished. Therefore, it was desirable
to make a qualitative and quantitative investigation of Blue
cheese flavor compounds and to study the effect of certain microorganisms
on Blue...