The primary purpose of this investigation was to determine the
conditions necessary for the preparation of cell-free extracts of
nodule bacteroids capable of catalysing the reduction of acetylene to
ethylene and nitrogen to ammonia. Initial investigations were conducted
to determine whether intact soybean root nodules would reduce
acetylene to ethylene...
In this dissertation, a new computational method for equality constrained nonlinear programming is developed. Specifically, problems of minimizing a nonlinear objective function f(x) subject to constraints h(x) = 0, where f is a scalar valued, x is an n-vector and h is a m-vector with m < n is considered....
Two spectrally different species of holotryptophanase
were obtained from fluorescence and circular dichroism
measurements. One is predominant in solutions containing
certain enzyme activating monovalent cations while the
other predominates in solutions of various inhibiting monovalent
cations. Relative magnitudes of the cation sensitive
fluorescent intensity maxima or circular dichroism
maxima are...
Monomer-tetramer association equilibrium and the oxygen
binding of the hemocyanin from Callianassa californiensis
were measured at a number of pH values and magnesium
ion concentrations. Magnesium binding of the hemocyanin
was also measured at several pH values. A thermodynamic
model, which satisfied the constraints from the experimental
results, was developed...