The glycoprotein, G, of infectious hematopoietic necrosis virus, IHNV,
was shown to be the viral protein which elicits and reacts with neutralizing
antibodies. The G protein also confers protective immunity on immunized
susceptible salmonid hosts to IHNV. The other three structural proteins of
IHNV, N, Ml, and M2, do not...
The development of an effective infectious
hematopoietic necrosis virus (IHNV) vaccine depends on a
better understanding of the antigenic structure and
variation among isolates. Three objectives to elucidate
this were: (1) to generate a panel of antigenic
derivatives of the IHNV glycoprotein by monoclonal
antibody-mediated selection of neutralization-resistant
variant viruses,...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...
Erythrocytic inclusion body syndrome (EIBS), a viral disease
of salmon, was investigated. Reliable artificial infections were
established in coho salmon (Oncorhynchus kisutch) via
intraperitoneal injection of homogenized kidney, spleen, and blood
from naturally infected animals. By examining the exposed fish
periodically for viral inclusion bodies and hematocrits, the disease
progression...
A survey of major bean (Phaseolus vulgaris) breeding programs and
seed producing areas in Idaho, Washington, and Oregon was conducted to
determine the occurrence of cucumber mosaic virus (CMV), recently reported
to be seedborne in beans. Some 259 plants, sampled from breeding
lines of diverse germplasm, were found by enzyme-linked...
DNA fragments of the gene for VP2, the major capsid protein of Infectious
Pancreatic Necrosis Virus (IPNV), were expressed in Escherichia coli and
characterized immunologically with antisera made to different serotypes of
IPNV. The entire VP2 gene was excised from the plasmid pUC19/A+SΔK and
fragmented with restriction enzyme Sau3A1. The...