The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
Chloroplast genomes of conifers are unusual in a
number of respects, including the possession of much
repetitive DNA. By studying the distribution and sequences
of dispersed repetitive DNA, I hoped to gain insight into
the mechanisms of chloroplast genome evolution.
I used restriction mapping and DNA sequencing to
characterize dispersed...
The genome of the multicapsid nuclear polyhedrosis virus of
Orgyia pseudotsugata (OpMNPV) was mapped by examining overlapping
Hindlll fragments from cosmid clones which had been constructed
from partial Hindlll digests of viral DNA. Five OpMNPV cosmid
clones containing fragments encompassing the entire OpMNPV genome
were hybridized to blots of DNA...
Complementary DNA (cDNA) clones were generated from the RNA genome
of infectious hematopoietic necrosis virus (IHNV) by using random DNA
oligomers to prime first strand synthesis. These clones were mapped to
their respective locations on the genome and used to determine the
nucleotide sequence of the nucleocapsid (N) gene and...
The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of
a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and
helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage
event catalyzed by HC-Pro...
Baculoviruses comprise a diverse group of pathogens
infectious for members of the insect orders Lepidoptera, Hymenoptera
and Diptera. Of the three subgroups, two (subgroup A, nuclear
polyhedrosis virus (NPV) and subgroup B, granulosis virus (GV) are
occluded in a crystalline protein structure while the remaining
subgroup (subgroup C, non-occluded virus)...
The prototypal poxvirus vaccinia is a cytoplasmic-replicating DNA
virus which contains a large genome with the capacity to encode 200
polypeptides. Tight temporal regulation coordinates the expression of
this large number of genes throughout a relatively short replication
cycle. As a first step towards identifying and understanding the
regulatory mechanisms...
The objective of this investigation was to evaluate the
possibility of developing high yielding wheat cultivars whose flour
would result in acceptable loaf volumes for non-traditional bread
wheat growing areas. Spring wheat germplasm employed included high
protein hexaploid derivatives from Triticum dicoccoides, and
selections from Argentina with good bread milling...