A sucrose density-gradient ultracentrifugation technique for
obtaining microsomes has been developed. This technique has
advantages over the classic differential centrifugation technique, in
that it is faster, is better suited to work with small quantities of
tissue, and offers the opportunity to work with a defined microsomal
sub-cellular fraction, rather than...
The mechanism(s) involved with intrauterine migration of the porcine embryo was investigated. Twenty-four gilts on Days 6, 9 and 12 of gestation (Day 0 = 1st day of estrus) were utilized to examine the relationship between myometrial activity and intrauterine migration. On Day 2, embryos were flushed from one oviduct...
NADPH-cytochrome P-450 reductase, an important enzyme in the
cytochrome P-450-dependent microsomal monooxygenase system, was
purified from insecticide-resistant (R) and -susceptible (S) house
flies (Musca domestica, L.) and from the black blow fly (Phormia
regina, Weigen). Proteolytic degradation to a P-450 - inactive form
of the reductase was reduced during the...