Stratified seeds of coastal Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. menziesii) were germinated, sown in soil, and seed coats and megagametophytes were removed at various stages of early seedling development. Yield and quality of DNA extracted from the megagametophytes were related to several morphological traits of the seedlings after
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"In 1954, Dr. Helge Irgens-Moller initiated a rangewide collection of seed and seedlings of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). Nearly 700 trees or stands were sampled in 10 western states, Canada, and Mexico. From 1957 to 1971, 639 of these collections were planted in the Hospital Tract Rangewide Source Archive...