Marker information in the new oilseed crop, meadowfoam, is limited. Molecular markers to facilitate meadowfoam breeding and cultivar improvement are not available. The knowledge of genetic relationships among recent germplasm is not known. The objectives of this study were (i) to gain an understanding of genetic diversity and relationship patterns...
Making quality and winterhardiness in barley are "ultimate" phenotypes composed of
component, quantitatively inherited traits. A 69-point genome map of the seven chromosomes of
barley was used, in conjunction with multi-environment phenotypes for grain yield and malting
quality, to determine the chromosome locations of quantitative trait loci (QTLs). A combined...
A series of experiments was performed to determine the feasibility of genomic
DNA-based methods for ecological and physiological studies of dominant bacteria from
complex natural systems. Two approaches, bacterial chromosomal painting (BCP) and
environmental genomics (EG), were evaluated for the potential to identify and enumerate
specific bacterial groups in situ...
Pulsed-field gel electrophoresis (PFGE) of the strains representing the 14 races of
U. hordei, the causal agent of covered smut on barley, revealed extensive polymorphism
in chromosome length and number. The purpose of this study was to determine by two
approaches the exact chromosome number for each U. hordei race,...
To help understand the mechanisms of conifer chloroplast genome evolution, we characterized the nucleotide sequences of Douglas-fir (Pseudotsuga menziesiz) and
Monterey pine (Pinus radiata) chioroplast DNA that are associated with a length
mutation hotspot and inversion. The chloroplast DNA ofthese two conifers are
characterized by shared large inversions that are...
The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa,
HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral
infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine
if P1 is necessary...
Basic biological information is stored in strings of nucleic acids (DNA, RNA) or amino acids
(proteins). Teasing out the meaning of these strings is a central problem of modern biology. Matching and
aligning strings brings out their shared characteristics. Although string matching is well-understood in the
edit-distance model, biological strings...
The level of polymorphisms of many biochemical and DNA markers are low in
cultivated sunflower (Helianthus annuus L.). The number of mapped public DNA
markers is limited. Molecular markers have not been developed for the most
important diseases of sunflower, such as downy mildew. The objectives of this
study were...
Viruses or viruslike particles of eukaryotic algae are ubiquitous in aquatic
habitats, however, suprisingly little is known about them. The research presented here
focused on one such virus which infects a multicellular filamentous brown alga of the
genus Feldmannia. Although preliminary studies had been performed on the genome
structure of...
The data used for the construction of genome maps is imperfect, therefore the mapping of a physically linear structure must take place in a very uneven feature space. As the number of genes to be ordered grows, it appears to be impractical to use exhaustive search techniques to find the...