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Filtering by: Creator Khanna, Satya Pal Remove constraint Creator: Khanna, Satya Pal Decade 2000-2009 Remove constraint Decade: 2000-2009 Degree Field Animal Science Remove constraint Degree Field: Animal Science

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  • Purification and characterization of recombinant calpain-5

    Creator:
    Wang, Mei-Chuan
    Abstract:
    Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....
    Resource Type:
    Dissertation
    Full Text:
    homology in Domain I (a long N-terminal region) and III (Pal homologous domain; PBH) (Sorimachi et al