The effect of increased manganese superoxide dismutase (Mn-SOD) and cytosolic ascorbate peroxidase (cAPX) on various abiotic stresses was studied using transgenic tomato (Lycopersicon esculentum) plants. Transformants were selected in vitro based on kanamycin resistance, confirmed by PCR and northern blot analyses. Northern blots showed an enhanced gene expression of Mn-SOD...
Pesticides are among the most pervasive environmental contaminants and they are an important potential risk for human health. Agricultural workers are constantly exposed to pesticide spray, drift and residues in the soil and foliage. Many agricultural pesticides are readily absorbed by the body, through contact with the skin, the respiratory...
Oxidative stress is recognized as an important underlying factor in the
pathogenesis of many degenerative diseases as well as normal senescence. The
free radicals, reactive oxygen species (ROS) and electrophiles produced during
oxidative stress are capable of modifying nucleic acids, lipids and proteins. There
are a variety of oxidative modifications...
The adduction of proteins and other biomolecules by electrophilic lipid peroxidation products such as 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), malondialdehyde (MDA) or acrolein (ACR) is thought to be an initiating and/or propagating factor in the pathophysiology of several diseases such as atherosclerosis, diabetes, Alzheimer's, Parkinson's and other age-related disorders. The identification...
Oxidative stress occurs when the balance between reactive oxygen species and antioxidant defense mechanisms in the body becomes tipped heavily in favor of the reactive oxygen species. Reactive oxygen species can be present in excess due to elevated O2 levels, radiation, infection, smoking, or even excessive exercise. It is also...
Approximately 12% of Americans do not consume the amount of zinc equal to the Estimated Average Requirement for zinc and could be at risk for marginal zinc deficiency. Zinc is an essential component of proteins in the defense against oxidative stress and DNA damage repair. Specifically in the prostate, zinc...
Despite the advances in surgery, physical therapy, and pharmaceutical agents, muscle
dysfunction (i.e., atrophy and weakness) continues to impair recovery from an anterior
cruciate ligament (ACL) injury and surgery. Ischemia-reperfusion injury during surgery
and the subsequent limb disuse are two events experienced by patients having ACL
surgery. Oxidative stress and...
Hallmarks of aging include the accumulation of aberrant proteins and a lower resistance to stresses. Because the endoplasmic reticulum (ER) functions to fold proteins and possesses unique stress sensing pathways, the central hypothesis of this dissertation is that the ER is a target of cellular aging and significantly underlies these...