Experiments were conducted to characterize the nongenomic effects of
progesterone (P4) on binding of oxytocin (OT) to the ovine oxytocin
receptor (OTR) and signal transduction. The dose-response relationship of
P4 to OT binding to the OTR in endometrium was examined. Progesterone
interfered with the binding of OT to the OTR...
The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...
New synthetic strategies have been investigated for nano-dimensioned
graphite particles or even isolated graphene sheets. One approach is to prepare
graphite intercalation compounds (GICs) with low layer charge density and
appropriate gallery height, followed by the colloidal dispersion or exfoliation of the
resulting GICs. A series of anions has been...
Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent signaling pathways in development and homeostasis. Grp1-associated scaffold protein (GRASP; also known as Tamalin) was first identified by our group, as a gene robustly induced...
Zinc is an essential mineral that is integral to many proteins and transcription factors that regulate key cellular functions. The normal prostate accumulates high concentrations of zinc; however, malignant tissues have significantly lower zinc levels. This unique relationship between zinc and the prostate has sparked interest in the role of...
This book is based on a project to completely annotate what is known about the function of the ~150 genes encoded by the genome of the most commonly investigated baculovirus, AcMNPV. Once the annotation was completed (summarized in Chapter 11), the material was organized into a series of chapters integrating...
Sweet whey powder is mainly used as an ingredient in foods and has a potential for increased use with the development of new foods. In view of the many whey powder producers, there is need to establish the quality of the whey powders currently on the market in terms of...
The effect of cosputtered catalyst on growth and alignment of carbon nanotubes (CNTs) grown by plasma enhanced chemical vapor deposition (PECVD) was investigated. Aligned CNTs were observed using a cosputtered catalytic metal deposited directly on boro-aluminosilicate glass. Catalytic metal alloys were sputtered directly onto the substrate using magnetron sputtering. Deposition...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...