Index Catalog // ScholarsArchive@OSU

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Creator:: Young, James Patrick
Abstract:: The ability to faithfully replicate DNA is dependent upon the maintenance and regulation of its precursors, the deoxyribonucleoside triphosphates. Enzymes encoded by the bacteriophage T4 have been widely used as models of biochemical processes. A body of evidence supports the concept that the bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Resource Type:: Dissertation

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Creator:: Howell, Meredith L.
Abstract:: Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project was to characterize the ribonucleotide reductase encoded by the orthopoxvirus, vaccinia. The first objective was to study the structural and functional features of the viral small subunit...
Resource Type:: Dissertation

Regulation of expression of four baculovirus genes and the immunocytochemical characterization of their products

Creator:: Gross, Christian Hans
Abstract:: Regulation of expression of three genes in the polyhedron envelope protein (PEP) gene region of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) was examined. These genes include open reading frame (ORF) 1 (encoding p21), ORF 2 (encoding gp16), and ORF 3 (encoding the polyhedron envelope protein). The effect of...
Resource Type:: Dissertation

Nucleoside diphosphokinase of Escherichia coli and its interactions with bacteriophage T4 proteins of DNA synthesis

Creator:: Ray, Nancy Bisset
Resource Type:: Dissertation

Biosyntheis of Blasticidin S : pathway and enzymes for the nucleoside formation and blastidic acid assembly

Creator:: Guo, Jincan
Resource Type:: Dissertation

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Creator:: Davis, Ralph Eugene, 1957-
Abstract:: Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
Resource Type:: Dissertation

Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

Creator:: Zhong, Lingxiu
Resource Type:: Dissertation

Exogenous gene expression from heterologous promoters in fish cell cultures

Creator:: Sharps, Angela
Abstract:: Cell culture systems have provided many insights into eukaryotic gene expression and other biochemical mechanisms. Since the cell represents the smallest living unit of any organism it provides a desirable in vitro system, allowing biochemical studies without the complex physiology of an entire animal. However, processes involving intracellular mechanisms, such...
Resource Type:: Masters Thesis

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

Creator:: Brown, D. W. (David W.), 1937-
Abstract:: Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these studies. First, the fluorescence anisotropy decay of bound ethidium was measured and was shown to reflect the rotational tumbling of the core particle through solution,...
Resource Type:: Dissertation

Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

Creator:: Varga Weisz, Patrick D.
Abstract:: Serum-free mouse embryo (SFME) cells are derived in medium in which serum is replaced with growth factors and other supplements. They display unusual properties. They do not lose proliferative potential or show gross chromosomal aberration upon extended culture, they depend on epidermal growth factor (EGF) for survival, and are reversibly...
Resource Type:: Dissertation

ScholarsArchive@OSU

Enzyme associations in deoxyribonucleotide biosynthesis : anti-idiotypic antibodies as probes for direct protein-protein interactions

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Regulation of expression of four baculovirus genes and the immunocytochemical characterization of their products

Nucleoside diphosphokinase of Escherichia coli and its interactions with bacteriophage T4 proteins of DNA synthesis

Biosyntheis of Blasticidin S : pathway and enzymes for the nucleoside formation and blastidic acid assembly

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

Exogenous gene expression from heterologous promoters in fish cell cultures

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

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