In this study, we developed a simple and efficient Bacillus subtilis genome editing method in which targeted gene(s) could be inactivated by single-stranded PCR product(s) flanked by short homology regions and in-frame deletion could be achieved by incubating the transformants at 42 degrees C. In this process, homologous recombination (HR)...
What constitutes a quality solution to an authentic task from industry? This study seeks to address this question through the examination of two expert solutions to an authentic engineering task used in the Chemical, Biological and Environmental Engineering curriculum at Oregon State University. The two solutions were generated by two...
Physical-Layer Network Coding (PNC) is a promising technique that has great potentials for improving the achievable data rates of end-to-end flows through higher packet transmission rates, thereby increasing the overall network throughput. In this thesis, we study the performance of the PNC transmission techniques for unidirectional end-to-end flows in multi-hop...
Current life cycle assessment tools are often deficient in assisting design for sustainable manufacturing efforts. Integrating an improved assessment method into a decision support framework will provide a means for designers and engineers to better understand the impacts of their decisions. A unit process modeling-based sustainability assessment method is presented...
Logging and forest thinning operations generate large amounts of residues in the form of small trees, branches and foliage that are usually left on the ground to decay or are burned. These resources are readily available, but it is uneconomical to remove them from the forest. The potential utilization of...
The factors that control the explosivity of silicic volcanoes are critical for hazard assessment, but are often poorly constrained for specific volcanic systems. Mount Hood, Oregon, is a somewhat atypical arc volcano in that it is characterized by a lack of large explosive eruptions over the entire lifetime of the...