Plasmid transformation of chlamydiae has created new opportunities to investigate host–microbe interactions during chlamydial infections; however, there are still limitations. Plasmid transformation requires a replicon derived from the native Chlamydia plasmid, and these transformations are species-specific. We explored the utility of a broad host-range plasmid, pBBR1MCS-4, to transform chlamydiae, with...
The primary goal of this dissertation is to improve the quality of nuclear data available to the nuclear science community. We propose to accomplish this by applying machine learning algorithms to the large number of available benchmark experiments and simulations, with the goal of determining which nuclear data have strong...
Taking long-term electrical measurements of a large number of neurons simultaneously is required for many modern neuroscience experiments. However, such experiments are currently limited by the shortcomings of traditional neurosensing technology. For example, there is a significant mechanical mismatch between rigid silicon probes and soft biological tissues. This mismatch can...
Oregon white oak, or Garry oak (Quercus garryana), has been adversely affected by land-use conversion, landscape fragmentation, and anthropogenic fire suppression. Its historical range has contracted significantly to the point where an estimated 1-5% remains intact. Natural regeneration is difficult due to a lack of seed sources and the physiological...
In this dissertation, I describe the experimental investigation of electron diffraction of neutral and ionic clusters doped into superfluid helium droplets. The project is aimed at contributing to the development of an overall approach called ‘serial single molecule electron diffraction imaging (ss-EDI), a potential approach to solve macromolecule structures bypassing...
Salmonid fish raised in hatcheries often have lower fitness (number of returning adult offspring) than wild fish when both spawn in the wild. Body size at release from hatcheries is positively correlated with survival at sea. So one explanation for reduced fitness is that hatcheries inadvertently select for trait values...
Autonomous agents that sense, decide, act, and coordinate effectively with each other are critical in many real-world domains such as autonomous driving, search and rescue missions, air traffic management, and underwater or deep space exploration. All such domains share a key difficulty: though high-level mission goals are clear to system...
For this project, we designed a soot modeling software to produce a full particle density function. This model uses the Direct Simulation Monte Carlo with a full conditioning particle selection method to capture soot nucleation and coagulation in a diffusion flame. We investigate the use of graphic processing units as...
Timber-concrete composite (TCC) solutions originated in the early twentieth century. There has been a renewed interest in TCC solutions in modern mass timber buildings, especially for floor systems. However due to lack of data, design standards, and code acceptance, TCC solutions require that new mass timber solutions be tested, and...
A critical component in diabetes management is diabetes self-management education (DSME). Additionally, poor self-management behavior and negative health outcomes are associated with participants that do not complete diabetes education (Gurdiacci et al., 2007). Despite the widespread use of DSME, many do not obtain the wide range of benefits these programs...