The first goal of this study was to evaluate the roles of different intracellular
proteolytic systems in accelerated skeletal muscle protein degradation. Total,
myofibrillar and non-myofibrillar proteolysis were determined in cultured L8/CID
muscle cells. Calpain proteolysis was assessed by over-expressed calpastatin inhibitory
domain (CD). Lysosomal proteolysis was determined in the...
Recombinant human calpain-5 was expressed in insect cells using a baculovirus system. The expressed calpain-5 was purified by both traditional chromatography and by affinity-column chromatography. Both methods yielded active protease. Calpain-5 displayed very limited hydrophobicity. This indicated that calpain-5 is not a membrane binding protein. Calpain-5 had pI of 8.3....
The overall objective of this research was to define the cellular basis underlying
heritable subfertility in roosters homozygous for the rose comb allele (R/R). Fertilization
in the hen is preceded by the ascension of motile sperm through the vagina and sperm
sequestration within sperm storage tubules (SST). The objective of...
Glucocorticoids are growth-inhibiting steroids. They have been reported to reduce
muscle growth by reducing protein synthesis. However, their actions on muscle protein
degradation remain equivocal. Glucocorticoids have been reported to transiently increase
muscle protein degradation, to not affect this process and to reduce muscle protein
degradation. Reasons for these conflicting...
Three studies during three consecutive summers were conducted
at the Squaw Butte Experiment Station to estimate relative and quantitative
forage intake of spring and fall calving cow-calf pairs on
summer range. In a drylot study (experiment 1) individual animal
consumption of fescue or meadow hay cut at 2- to 10-day...
We conducted two experiments evaluating perennial ryegrass straw as a forage source for ruminants. Experiment 1 evaluated digestion and physiological variables in steers offered perennial ryegrass straw containing increasing levels of lolitrem B. Sixteen ruminally cannulated Angus X Hereford steers (231 ± 2 kg BW) were blocked by weight and...
The major purpose of this research was to characterize partially degraded proteins
appearing in the ocular lens during aging and cataract, and to identify the responsible
proteolytic activities. This research is significant, because increased protein
degradation is associated with lens opacification and cataract. Determining the sites
where lens proteins become...