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Characterization of the Zea mays ssp. mays TOUSLED-like kinases

Creator:: Owusu, Ethel Owusuwaa
Abstract:: This dissertation describes the cloning and characterization of the TOUSLEDlike kinases genes of maize (ZmTLKs). The TOUSLED-like kinases (TLKs) are a conserved family of nuclear Ser/Thr kinases in higher eukaryotes. The maize genome has three TOUSLED-like kinase genes (ZmTLK1, ZmTLK2, and ZmTLK3). Based upon sequence similarity, the ZmTLKs are divided...
Resource Type:: Dissertation

Characterization of different forms of lysophospholipase in barley

Creator:: Lundgard, Robert
Abstract:: During barley germination, α-amylase and other hydrolases are synthesized in the aleurone layer and secreted into the starchy endosperm. As starch is degraded by amylases, lysophospholipids are released from inclusion complexes with the starch and undergo hydrolysis by a lysophospholipase (LPL) activity. Studies with embryo-free half seeds and isolated aleurone...
Resource Type:: Dissertation
Full Text:: ; ultrastructural study. J. Expt. Bot. 27: 759-768. 33. Evins, W.H. (1971) Enhancement of polyribosome formation

Rapid kinetic studies of thymidylate synthase

Creator:: Mittelstaedt, Denice
Resource Type:: Dissertation
Full Text:: 25° 768 ± 371 610 ± 400 19.0 21.5 t 3.2 30° dUMP 233 ± 15 81.3 ± 18 0.9 2.5 t 0.7 10° 188 t 81 62

Protein methylation at sites of blood vessel injury

Creator:: Weber, Darin J.
Abstract:: Blood vessel injury was found to release intracellular pools of protein D-aspartyl/L-isoaspartyl carboxyl methyltransferase (PIMT) into the extracellular milieu, where it became trapped. Trapped PIMT was able to utilize radiolabeled S-adenosyl-L-methionine (AdoMet) introduced into the circulation to methylate blood vessel proteins containing altered aspartyl residues specifically at the site of...
Resource Type:: Dissertation
Full Text:: methyltransferase (EC 2.1.1.77) purified from rabbit erythrocytes (Gilbert et al. 1988) was added to the assay

Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity

Creator:: Thomas, Nicholas O.
Abstract:: Over the past century, life expectancy in the United States has dramatically increased leading to an increasingly aging population with people reaching, and spending more years in ‘old age’. While this unprecedented shift in population demographics represents great strides for humanity, it is not without cost. One consequence of longer...
Resource Type:: Dissertation

Cytoskeletal regulation in cell motility and invasion

Creator:: Jang, Hyo Sang
Abstract:: Cell motility and invasion are important for development, immunity, wound healing, and tumor cell metastasis. Cells on two dimensional substrates migrate in three steps: protrusion of the front end, translocation of the cell body, and retraction of the rear end. For cells to migrate efficiently, these steps need to be...
Resource Type:: Dissertation

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Creator:: Howell, Meredith L.
Abstract:: Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project was to characterize the ribonucleotide reductase encoded by the orthopoxvirus, vaccinia. The first objective was to study the structural and functional features of the viral small subunit...
Resource Type:: Dissertation

Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor

Creator:: Vogel, Walter Kevin
Resource Type:: Dissertation

Regulation of phosphofructokinase by reversible inactivation

Creator:: Zhao, Zhizhuang
Abstract:: The interaction of skeletal muscle phosphofructokinase with a variety of acidic proteins including calmodulin and troponin C and with nucleic acids (RNA and DNA) is manifest in a reversible, time-dependent loss of catalytic activity. The inactivation is affected by a number of factors. Substrates (fructose-6-phosphate and ATP), positive effectors (fructose-2,6-bisphosphate,...
Resource Type:: Dissertation
Full Text:: phosphofructokinase (PFK, ATP:D-fructose- 6-phosphate - 1 - phosphotransferase, EC 2. 7. 1. 11) is a key glycolytic

Toxicity and in vitro metabolism of Senecio jacobaea pyrrolizidine alkaloids in the rat and mouse

Creator:: Ramsdell, Howard Scott
Abstract:: Senecio jacobaea (tansy ragwort) is a common weed in the Pacific Northwest that contains toxic pyrrolizidine alkaloids (PAs). Jacobine, the major PA in Senecio jacobaea, was incubated with rat liver microsomes and metabolites were isolated by high performance liquid chromatography (HPLC) and identified by mass spectrometric analysis. Metabolites included a...
Resource Type:: Dissertation

ScholarsArchive@OSU

Characterization of the Zea mays ssp. mays TOUSLED-like kinases

Characterization of different forms of lysophospholipase in barley

Rapid kinetic studies of thymidylate synthase

Protein methylation at sites of blood vessel injury

Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity

Cytoskeletal regulation in cell motility and invasion

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor

Regulation of phosphofructokinase by reversible inactivation

Toxicity and in vitro metabolism of Senecio jacobaea pyrrolizidine alkaloids in the rat and mouse

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