Index Catalog // ScholarsArchive@OSU

Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide

Creator:: Tiffany, Thomas Oliver
Resource Type:: Dissertation
Full Text:: reagents used for the preparation of ribonuclease-S were reagent grade chemicals purchased from Baker

Studies on the interaction of polylysine with deoxyribonucleic acid

Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation

Exploring Protein Structure: Seeing the Forest and the Trees

Creator:: Brereton, Andrew E.
Abstract:: Life on Earth intimately depends on the function of countless proteins. For the majority of studied proteins, function absolutely depends on conformation (i.e. 3-dimensional shape in solution). The exact nature of how a protein goes from an unfolded linear polypeptide chain to an organized folded molecule is still not known,...
Resource Type:: Dissertation

An electrophoretic investigation of nucleoprotein organization in yeast chromatin

Creator:: Szent-Györgyi, Christopher Stephan
Abstract:: I have investigated nucleoprotein organization in chromatin of the yeast, Saccharomyces cerevisiae. My approach has been to prepare insoluble, minimally per turbed chromatin from a mitochondrial DNA-free strain of yeast, using a number of new techniques, and then digest this substrate with micrococcal nuclease. The soluble products are electrophoresed on...
Resource Type:: Dissertation

The interaction of polylysine with DNA as studied by polarization of flurescence

Creator:: Evett, Jay Fredrick
Abstract:: A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
Resource Type:: Dissertation
Full Text:: dissociation runs were carried out by adding dry salt (reagent grade, J. T. Baker Chemical Co. ) to a sample

The purification and characterization of the fructose-1, 6-diphosphate aldolase from Bacillus stearothermophilus (NCA 2184)

Creator:: Quinn, Richard Paul
Abstract:: The thermophilic aldolase from Bacillus stearothermophilus (NCA 2184) has been purified to a specific activity of 45.5 μ-moles of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme, representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation and by a specific...
Resource Type:: Dissertation
Full Text:: Whatman DE-23. Ethylenediaminetetraacetic acid (EDTA), reagent grade, was purchased from the J. T. Baker

An integrative approach to understanding pyoluteorin biosynthesis in the biological control bacterium Pseudomonas fluorescens Pf-5

Creator:: Nowak-Thompson, Brian
Resource Type:: Dissertation
Full Text:: , excluding humans (Cook and Baker, 1983). Biological control of plant pathogens can be accomplished through

Purification and characterization of an alkaline phosphatase from Halobacterium salinarium

Creator:: McParland, Reginald Harry
Abstract:: The halophilic alkaline phosphatase of Halobacterium salinarium has been purified to a specific activity of 3,000-3,200 units per mg of protein. The purification scheme consisted of acetone and ammonium sulfate fractionations, and molecular sieving and adsorption chromatography on Sephadex G -50. The enzyme was stabilized during purification by the presence...
Resource Type:: Dissertation
Full Text:: , California. All other salts used for cultivation and enzyme work were reagent grade purchased from Baker

Vacuum ultraviolet absorption studies of model sugar compounds

Creator:: Dickinson, Helen Rose
Abstract:: Vacuum ultraviolet absorption spectra of 13 model sugar compounds were measured in the energy range of 50 to 80 kK. The model sugars included three simple alcohols, four simple ethers, four cyclic alcohol-ether compounds, and two dioxanes. All spectra show transition bands in the energy region 50 to 65 kK....
Resource Type:: Dissertation
Full Text:: Tetrahydrofuran Baker Analyzed Distillation O Reagent H CCH-OH 2/ \ 1H C CH 2 0" 2 3-Hydr oxytetrahydrofuran

The effects of hexachlorophene on mitochondrial and artificial membrane systems

Creator:: Ramberg, Donald Allen
Abstract:: Hexachlorophene (HCP; 2,2'-methylenebis-(3,4,6-trichlorophenol)) was developed as an antibacterial agent but was also found to induce toxic effects in various tissues in animals and humans. Changes were found in myelinated nerve fibers and cerebral white matter. Erythrocytes are hemolysed in vitro. Oxidative phosphorylation is uncoupled by HCP at concentrations less than...
Resource Type:: Dissertation
Full Text:: '-methylenebis-(3,4,6- trichlorophenol)] as an antibacterial agent (Gump and Walter, 1962, 1963, 1964) led to

ScholarsArchive@OSU

Some physical studies on ribonuclease-S and its component parts, S-protein and S-peptide

Studies on the interaction of polylysine with deoxyribonucleic acid

Exploring Protein Structure: Seeing the Forest and the Trees

An electrophoretic investigation of nucleoprotein organization in yeast chromatin

The interaction of polylysine with DNA as studied by polarization of flurescence

The purification and characterization of the fructose-1, 6-diphosphate aldolase from Bacillus stearothermophilus (NCA 2184)

An integrative approach to understanding pyoluteorin biosynthesis in the biological control bacterium Pseudomonas fluorescens Pf-5

Purification and characterization of an alkaline phosphatase from Halobacterium salinarium

Vacuum ultraviolet absorption studies of model sugar compounds

The effects of hexachlorophene on mitochondrial and artificial membrane systems

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