Index Catalog // ScholarsArchive@OSU

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Creator:: Weber, Shane Crawford
Abstract:: I examined, in three separate studies, the active chromatin structure of the yeast, Saccharomyces cerevisiae. Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted by 0.25...
Resource Type:: Dissertation
Full Text:: authorship: J. R. Davie, C. A. Saunders, J. M. Walsh and S. C. Weber. In accordance with graduate school

Protein methylation at sites of blood vessel injury

Creator:: Weber, Darin J.
Abstract:: Blood vessel injury was found to release intracellular pools of protein D-aspartyl/L-isoaspartyl carboxyl methyltransferase (PIMT) into the extracellular milieu, where it became trapped. Trapped PIMT was able to utilize radiolabeled S-adenosyl-L-methionine (AdoMet) introduced into the circulation to methylate blood vessel proteins containing altered aspartyl residues specifically at the site of...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE THESIS OF Darin J. Weber for the degree of

The 2-μm Plasmid of Saccharomyces cerevisiae; Partial Characterization of a Eukaryotic Minichromosome

Creator:: Zsebo, Krisztina Maria
Resource Type:: Masters Thesis
Full Text:: work. Purified HMGa was supplied by Shane Weber. DNA from clone pYlrAl2 was supplied by Dr. Tom Petes

The renaturation of bovine muscle pyruvate kinase

Creator:: Porter, David Hamilton
Resource Type:: Dissertation
Full Text:: (Weber, 1969; Ibsen and Trippet, 1973,1974; Cardenas et al., 1975b). For rabbit and rat type M the

Pyruvate kinase isozymes in beef and chicken

Creator:: Strandholm, John Jeffrey, 1945-
Resource Type:: Dissertation
Full Text:: for privacyAbstract approved: Robert D. Dy on Redacted for privacy Janet M. Cardenas At least three

The effect of hexachlorophene and related compounds on torula yeast glucose-6-phosphate dehydrogenase and some other dehydrogenase enzymes

Creator:: Wang, Jun-Lan
Abstract:: Low concentrations of hexachlorophene (HCP) inhibit a number of pyridine nucleotide-linked dehydrogenase enzymes. The I₅₀ HCP concentrations were 105 μM for pig heart isocitrate dehydrogenase (ICD), 65 μM for horse liver alcohol dehydrogenase, 39 μM for torula yeast glucose-6-phosphate dehydrogenase (G6PD), 6.0 μM for beef heart malate dehydrogenase, and 1.6...
Resource Type:: Dissertation
Full Text:: K the apparent product of n dissociation constants K. enzyme-inhibitor dissociation constant K m

Studies on the interaction of polylysine with deoxyribonucleic acid

Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation
Full Text:: reported in the literature to be irre- versible at low ionic strength, i.e. 0.0 M to about 0.4-0.8 M NaCl

Factors affecting the activation of rabbit muscle phosphofructokinase by actin

Creator:: Kuo, Huey-ju
Abstract:: The consistent application of phosphatase inhibitors and a novel final purification step using a connected series of DE-51, DE-52, and DE-53 anion exchange chromatography columns facilitate the preparation of electrophoretically homogeneous sub-pupulations of rabbit muscle phosphofructokinase which differ in their catalytic properties and endogenous covalent phosphate contents. A band of...
Resource Type:: Dissertation
Full Text:: and ADP. Fructose 6-phosphate + ATP ---771.)Fructose 1,6-bisphosphate + ADP + H. M PFK g The

Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

Creator:: Malencik, Dean Anthony
Abstract:: Fluorescence polarization measurements on beef liver glutamate dehydrogenase conjugated with pyrene butyric acid demonstrate that the association of the enzyme is adequately described by a reversible indefinite association with a single equilibrium constant. The data obtained with protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7....
Resource Type:: Dissertation
Full Text:: protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7. 6, are consistent with a

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

Creator:: Brown, D. W. (David W.), 1937-
Abstract:: Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these studies. First, the fluorescence anisotropy decay of bound ethidium was measured and was shown to reflect the rotational tumbling of the core particle through solution,...
Resource Type:: Dissertation
Full Text:: was raised from 10 mM to 0.35 M, with further increases being observed only above 0.65 M; we term

ScholarsArchive@OSU

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Protein methylation at sites of blood vessel injury

The 2-μm Plasmid of Saccharomyces cerevisiae; Partial Characterization of a Eukaryotic Minichromosome

The renaturation of bovine muscle pyruvate kinase

Pyruvate kinase isozymes in beef and chicken

The effect of hexachlorophene and related compounds on torula yeast glucose-6-phosphate dehydrogenase and some other dehydrogenase enzymes

Studies on the interaction of polylysine with deoxyribonucleic acid

Factors affecting the activation of rabbit muscle phosphofructokinase by actin

Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

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