Search Constraints

Start Over
Filtering by: Subject DNA-protein interactions Remove constraint Subject: DNA-protein interactions Degree Field Biochemistry and Biophysics Remove constraint Degree Field: Biochemistry and Biophysics

Search Results

  • The mechanism of interaction of the linker histone with DNA and nucleosomes

    Creator:
    Ellen, Thomas Patrick
    Abstract:
    This dissertation examines the interaction of the linker histone with DNA and with nucleosomes. The first goal of the project was to characterize the interaction of the linker histone with DNA. Three factors previously reported to influence the linker histone's interaction with DNA were examined: ratio of linker histone to...
    Resource Type:
    Dissertation
    Full Text:
    Mechanism of Interaction of the Linker Histone with DNA and Nucleosomes. Abstract approved: This

  • Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

    Creator:
    Bernard, Mark Aguirre
    Abstract:
    Although the Escherichia coli host has almost all of the enzymes necessary to synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes expressed early in infection encode enzymes for de novo DNA precursor biosynthesis and salvage from degraded host DNA. Eight early enzymes and two host enzymes comprise the...
    Resource Type:
    Dissertation
    Full Text:
    interaction involving T4 DNA polymerase (gene 43 protein) and T4 ss-DNA binding protein (gene 32 protein) 101

  • Characterization of the Escherichia coli uracil-DNA glycosylase- inhibitor protein interaction

    Creator:
    Bennett, Samuel E.
    Resource Type:
    Dissertation
    Full Text:
    of the Escherichia coli Uracil-DNA Glycosylase Inhibitor Protein Interaction. Abstract approved

  • Organization of the T4 dNTP synthetase complex at DNA replication sites

    Creator:
    Kim, JuHyun
    Abstract:
    With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
    Resource Type:
    Dissertation
    Full Text:
    , the single-stranded DNA binding protein encoded by gene 32, plays a role in recruiting enzymes of

  • The asymmetric binding of Actinomycin D to DNA hexamers

    Creator:
    Ivancic, Monika
    Abstract:
    The solution structure determination of DNA molecules has been an important part of structural biology. NMR solution structures are a complement to structures solved via X-ray crystallography; the two methods are the only ways of obtaining three dimensional coordinates of macromolecules. Because of the nature of the molecule, the solution...
    Resource Type:
    Dissertation
    Full Text:
    transcribed into RNA. RNA then commuricates the genetic information to ribosomes for protein synthesis. DNA

  • Protein Disorder in the Evolution of Dynein Regulation

    Creator:
    Jie, Jing
    Abstract:
    Intrinsically disordered proteins (IDP) are a class of proteins that lack a three-dimensional structure and their prevalence and diverse functions in the cell have only been discovered relatively recently. The intermediate chain (IC) subunit of the microtubule motor protein complex dynein contains an N-terminal disordered region, N-IC, which is central...
    Resource Type:
    Dissertation
    Full Text:
    , and the DNA damage repair protein Rad50 (Mark et al., 6 2005; Uversky et al., 2008

  • Characterization of the Structure and Dynamics of the Intrinsically Disordered Dynein Intermediate Chain and its Interactions with Regulatory Binding Partners

    Creator:
    Morgan, Jessica L.
    Abstract:
    Cells represent microcosms of spatial and temporal structural organization, with the achievement of internal spatial organization relying upon a collection of macromolecular motor complexes to transport and localize components throughout the cell. Cytoplasmic dynein is one such motor complex, and is the principal ATP-dependent motor for retrograde transport along microtubules...
    Resource Type:
    Dissertation
    Full Text:
    ) DNA-binding domain of the CREB (cyclic AMP response element-binding protein) transcription factor

  • Protein-protein interactions in the bacteriophage T4 dNTP synthetase complex

    Creator:
    Shen, Rongkun
    Abstract:
    The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
    Resource Type:
    Dissertation
    Full Text:
    juxtaposed with the DNA replication apparatus ……… 49 1.6 Protein-protein interaction model identified